Home >> Antibodies >> Creatine Phosphokinase-BB (CK-BB)

Creatine Phosphokinase-BB (CK-BB)

Mouse Monoclonal Antibody [Clone CKBB/6565]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Western blot analysis of HEK-293 cell lysate using CKBB Mouse Monoclonal Antibody (CKBB/6565).

Western blot analysis of Y79 cell lysate using CKBB Mouse Monoclonal Antibody (CKBB/6565).

Formalin-fixed, paraffin-embedded human kidney stained with CKBB Mouse Monoclonal Antibody (CKBB/6565).

Formalin-fixed, paraffin-embedded human brain stained with CKBB Mouse Monoclonal Antibody (CKBB/6565).

Western blot analysis of HEK-293 cell lysate using CKBB Mouse Monoclonal Antibody (CKBB/6565).

Western blot analysis of Y79 cell lysate using CKBB Mouse Monoclonal Antibody (CKBB/6565).

Formalin-fixed, paraffin-embedded human kidney stained with CKBB Mouse Monoclonal Antibody (CKBB/6565).

Formalin-fixed, paraffin-embedded human brain stained with CKBB Mouse Monoclonal Antibody (CKBB/6565).

Product Details

Synonyms

BCK; Brain creatine kinase; Ckb; Creatine kinase B chain, Creatine kinase B-type; Creatine Kinase BB Isoenzyme; Creatine phosphokinase BB; Epididymis luminal protein 211; Epididymis secretory protein Li 29; HEL 211

Positive Control

SH-SY5Y or HEK-293 cells. Human cerebellum.

Known Applications & Suggested Dilutions

ELISA, Western Blot, IHC-P More Details

ELISA (For coating, order antibody without BSA)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human full-length CKB protein

Cellular Localization

Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

42kDa

Specificity & Comments

Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.