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Home >> Antibodies >> Clostridium difficile (Toxin A)

Clostridium difficile (Toxin A)

Mouse Monoclonal Antibody [Clone 253/422]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00

Product Details

Synonyms

Clostridium difficile; C. difficile; tcdA; toxA

Positive Control

Clostridium difficile extract or infected cells. Tissue.

Known Applications & Suggested Dilutions

ELISA, IF         More Details

ELISA (For coating use Ab at 1-5ug/ml, order Ab without BSA)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

C. difficile Toxin A

Cellular Localization

Cytoplasmic (Secreted)

Species Reactivity

Clostridium difficile

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

308kDa (Toxin A)

Specificity & Comments

This MAb reacts with C. difficile Toxin A, but not with Cholera subunit a, Cholera toxin, Pseudomonas aeruginosa exotoxin A, H-LT, P-LT. Clostridium difficile is a major nosocomial pathogen that causes antibiotic- associated colitis. Clostridium difficile mediates inflammatory diarrhea by releasing two large protein enterotoxins (Toxin A and Toxin B) that are able to disrupt intestinal epithelial cells via their transferase activity and ability to monoglucosylate members of the Rho family. Clostridium difficile Toxin A is a toxin that is composed of 39 repeats that are responsible for binding to intestinal epithelial cell surface carbohydrates. Clostridium difficile Toxin A causes significant apoptosis of colonocytes which contributes to the formation of ulcers and pseudo-membranes in a pathway that involves p38-dependent activation of p53 and induction of p21, leading to cytochrome c release and caspase-3 activation through Bak activation.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Kim, H., et al. 2005. Gastroenterology 129: 1875-1888.

Bioinformatics

  •  SwissProt: P16154
NBT00202