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CD59 / Complement Regulatory Protein / Protectin

Recombinant Rabbit Monoclonal Antibody [Clone MACIF/7021R]

Printable

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Bioinformatics

SDS-PAGE Analysis Purified CD59 Recombinant Rabbit Monoclonal Antibody (MACIF/7021R). Confirmation of Purity and Integrity of Antibody.

IHC analysis of formalin-fixed, paraffin-embedded human cerebellum. Cytoplasmic and membranous staining using MACIF/7021R at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.

IHC analysis of formalin-fixed, paraffin-embedded human kidney. Cytoplasmic and membranous staining using MACIF/7021R at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human spleen stained with CD59 Recombinant Rabbit Monoclonal Antibody (MACIF/7021R). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human tonsil stained with CD59 Recombinant Rabbit Monoclonal Antibody (MACIF/7021R). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis Purified CD59 Recombinant Rabbit Monoclonal Antibody (MACIF/7021R). Confirmation of Purity and Integrity of Antibody.

IHC analysis of formalin-fixed, paraffin-embedded human cerebellum. Cytoplasmic and membranous staining using MACIF/7021R at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.

IHC analysis of formalin-fixed, paraffin-embedded human kidney. Cytoplasmic and membranous staining using MACIF/7021R at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human spleen stained with CD59 Recombinant Rabbit Monoclonal Antibody (MACIF/7021R). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human tonsil stained with CD59 Recombinant Rabbit Monoclonal Antibody (MACIF/7021R). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

20kDa homologous restriction factor (HRF20); Complement regulatory protein; Human leukocyte antigen MIC11; MAC-inhibitory protein (MACIP); Membrane attack complex inhibition factor (MACIF); Membrane inhibitor of reactive lysis; MIRL; MSK21; Protectin; T cell activating protein

Positive Control

Daudi, K562, Jurkat or Raji cells. Human lymphocytes. Human lymph node or tonsil.

Known Applications & Suggested Dilutions

IHC-P More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human full-length CD59 protein

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG, kappa

Mol. Weight of Antigen

20kDa

Specificity & Comments

Reacts with human CD59, a 20kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein (Workshop VI; Code N-L036). CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This MAb recognizes CD59 transfected cells. It is useful for study on GPI-anchored proteins, PNH and CD59 functions.