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Home >> Antibodies >> CD45RB (B-Cell Marker)

CD45RB (B-Cell Marker)

Mouse Monoclonal Antibody [Clone PTPRC/1132]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RB Monoclonal Antibody (PTPRC/1132).

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Formalin-fixed, paraffin-embedded human Lymphoma stained with CD45RB Monoclonal Antibody (PTPRC/1132).

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Western Blot Analysis of Daudi cell lysate using CD45RB Monoclonal Antibody (PTPRC/1132).

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Western Blot Analysis of human Spleen tissue lysates using CD45RB Monoclonal Antibody (PTPRC/1132).

Product Details

Synonyms

B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Positive Control

Ramos, U-698 or GA-10 cells. Spleen and Tonsil.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human B-lymphocytes

Cellular Localization

Cell surface and cytoplasmic

Species Reactivity

Human. Does not react with Rat or monkey.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

180-220kDa

Specificity & Comments

CD45R, also designated CD45 and PTPRC, is identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. West, K.P., et al. 1986. The demonstration of B-cell, T-cell and myeloid antigens in paraffin sections. J. Pathol. 150: 89-101.
  2. Streuli, M., et al. 1987. Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens. J. Exp. Med. 166: 1548-1566.

Bioinformatics

  •  Entrez Gene ID: 5788
  •  Gene Symbol: PTPRC
  •  SwissProt: P08575
  •  Chromosome Location: 1q31.3
  •  Unigene: 654514
NBT01132