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Home >> Antibodies >> CD3e (T-Cell Marker)

CD3e (T-Cell Marker)

Mouse Monoclonal Antibody [Clone PC3/188A]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Tonsil stained with CD3e Mouse Monoclonal Antibody (PC3/188A).

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Western Blot Analysis of human Thymus lysate using Purified CD3e Mouse Monoclonal Antibody (PC3/188A).

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SDS-PAGE Analysis Purified CD3e Mouse Monoclonal Antibody (PC3/188A). Confirmation of Integrity and Purity of Antibody.

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Western Blot Analysis of human Jurkat cell lysate using CD3e Mouse Monoclonal Antibody (PC3/188A).

Product Details

Synonyms

CD 3E, CD3 epsilon, CD3 TCR complex, CD3E, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex

Positive Control

Jurkat cells. Tonsil and lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml for 60 minutes at RT)
Optimal dilution for a specific application should be determined.

Immunogen

A synthetic peptide corresponding to amino acids 156-168 of the cytoplasmic domain of human CD3-ε chain

Cellular Localization

Cell Surface and Cytoplasmic

Species Reactivity

Human. Rat. Guinea Pig. Rabbit. Pig. Horse. Cow. Chicken.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

20kDa

Specificity & Comments

Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Cibull ML et. al. Histopathology, 1989, 15(6):599-605.
  2. Mason DY et. al. Journal of Clinical Pathology, 1989, 42(11):1194-200.

Bioinformatics

  •  Entrez Gene ID: 916
  •  Gene Symbol: CD3E
  •  SwissProt: P07766
  •  Chromosome Location: 11q23.3
  •  Unigene: 3003
NBT01479