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Home >> Antibodies >> CD276 / B7-H3

CD276 / B7-H3

Mouse Monoclonal Antibody [Clone B7H3/4345]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with CD276 / B7-H3 Mouse Monoclonal Antibody (B7H3/4345) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

img

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with CD276 / B7-H3 Mouse Monoclonal Antibody (B7H3/4345) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD276 / B7-H3 Monospecific Mouse Monoclonal Antibody (B7H3/4345).

Product Details

Synonyms

4Ig B7 H3; B7 homolog 3; B7-H3; B7H3; B7RP-2; CD276; CD276 antigen; CD276 molecule; Costimulatory molecule

Positive Control

Human tonsil, placenta, hepatocellular carcinoma or lung carcinoma.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa100-300) of human CD276 protein (exact sequence is proprietary)

Cellular Localization

Cytoplasm. Cell surface.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

B7-H3 isoforms: 57/34/53/57 kDa; glycosylated B7-H3: 90-110 kDa

Specificity & Comments

T cell activation and immune function are regulated by the innate immune system through positive and negative costimulatory molecules. One such molecule, B7-H3 (B7-homolog 3, also designated B7RP-2) belongs to the B7 immunoglobulin superfamily. Soluble B7-H3 binds a putative receptor on activated T-cells that is distinct from CD28, CTLA-4, ICOS and PD-1. Widely expressed on nonlymphoid tissues, B7-H3 costimulates proliferation of both CD4+ and CD8+ T cells. The ability of B7-H3 to stimulate Th1 and cytotoxic-T cell responses suggest that it may have antitumor activity. B7-H3 interactions may play a role in regulating cell-mediated immune responses against cancer, implicating B7-H3 as a potential therapeutic tool.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Liu, F., et al. 2015. B7-H3 promotes cell migration and invasion through the Jak2/Stat3/MMP9 signaling pathway in colorectal cancer. Mol. Med. Rep. 12: 5455-5460.

Bioinformatics

  •  Entrez Gene ID: 80381
  •  Gene Symbol: CD276
  •  SwissProt: Q5ZPR3
  •  Chromosome Location: 15q24.1
  •  Unigene: 77873