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CD276 / B7-H3 Antibody [B7H3/4345]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
80381-MSM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
80381-MSM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
80381-MSM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

T cell activation and immune function are regulated by the innate immune system through positive and negative costimulatory molecules. One such molecule, B7-H3 (B7-homolog 3, also designated B7RP-2) belongs to the B7 immunoglobulin superfamily. Soluble B7-H3 binds a putative receptor on activated T-cells that is distinct from CD28, CTLA-4, ICOS and PD-1. Widely expressed on nonlymphoid tissues, B7-H3 costimulates proliferation of both CD4+ and CD8+ T cells. The ability of B7-H3 to stimulate Th1 and cytotoxic-T cell responses suggest that it may have antitumor activity. B7-H3 interactions may play a role in regulating cell-mediated immune responses against cancer, implicating B7-H3 as a potential therapeutic tool.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Research Areas
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Membrane
Gene Name
Positive Control
hepatocellular carcinoma or lung carcinoma., Human tonsil, Placenta
Immunogen
Recombinant fragment (around aa100-300) of human CD276 protein (exact sequence is proprietary)
Alternate Names
CD276 antigen, 4Ig-B7-H3, B7 homolog 3, Costimulatory molecule, 4Ig B7 H3; B7 homolog 3; B7-H3; B7H3; B7RP-2; CD276; CD276 antigen; CD276 molecule; Costimulatory molecule

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
B7H3/4345
Chromosome Location
15q24.1
Mol. Weight of Antigen
B7-H3 isoforms: 57/34/53/57 kDa; glycosylated B7-H3: 90-110 kDa

Functions

  • May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.

Key References

  • Liu, F., et al. 2015. B7-H3 promotes cell migration and invasion through the Jak2/Stat3/MMP9 signaling pathway in colorectal cancer. Mol. Med. Rep. 12: 5455-5460.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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