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Home >> Antibodies >> CD25 / IL2RA (Activated Lymphocyte Marker)

CD25 / IL2RA (Activated Lymphocyte Marker)

Mouse Monoclonal Antibody [Clone IL2RA/2394]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Formalin-fixed, paraffin-embedded human tonsil stained with CD25 Mouse Monoclonal Antibody (IL2RA/2394).

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Formalin-fixed, paraffin-embedded human tonsil stained with CD25 Mouse Monoclonal Antibody (IL2RA/2394).

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Formalin-fixed, paraffin-embedded human tonsil stained with CD25 Mouse Monoclonal Antibody (IL2RA/2394).

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SDS-PAGE Analysis Purified CD25 Mouse Monoclonal Antibody (IL2RA/2394). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing >19,000 full-length human proteins using CD25 Mouse Monoclonal Antibody (IL2RA/2394)

Product Details

Synonyms

IL2 Receptor alpha; IL2R alpha chain; IL2R; IL2R subunit alpha IL2RA; Interleukin-2 receptor subunit alpha; Ly43; p55; p55 chain; T Cell Growth Factor Receptor; TAC antigen; TCGFR

Positive Control

PHA-stimulated human lymphocytes. Human lymph node or tonsil

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human IL2RA protein fragment (around aa 42-183) (exact sequence is proprietary)

Cellular Localization

Cell surface. Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

55kDa

Specificity & Comments

Recognizes a protein of 55kDa, identified as CD25. It is expressed on activated T- and B-cells and activated monocytes/macrophages. With respect to lymphomas, CD25 is present on malignant cells of Hodgkin s disease, HTLV-1+ adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R  chain, p75) and CD132 (common  chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. This MAb recognizes the epitope B, which is located at residue 3-104 of CD25 and doe not block IL-2 binding to CD25.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. T.A. Waldmann, et al, (1989) Annu Rev Biochem 58: 875-911.

Bioinformatics

  •  Entrez Gene ID: 3559
  •  Gene Symbol: IL2RA
  •  SwissProt: P01589
  •  Chromosome Location: 10p15-p14
  •  Unigene: 231367