Learn about our comprehensive antibody validation methods to ensure monospecificity.  Antibody Validation>>

Printable Datasheet

CD25 / IL2RA (Activated Lymphocyte Marker) Antibody [143-13]

In Stock
Catalog Number Formulation Size Price
3559-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3559-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3559-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis of Purified CD25 Mouse Monoclonal Antibody (143-13). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis of Purified CD25 Mouse Monoclonal Antibody (143-13). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml

Summary

Recognizes a protein of 55kDa, identified as CD25. It is expressed on activated T- and B-cells and activated monocytes/macrophages. With respect to lymphomas, CD25 is present on malignant cells of Hodgkin's disease, HTLV-1+ adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R chain, p75) and CD132 (common chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. This MAb recognizes the epitope B, which is located at residue 3-104 of CD25 and doe not block IL-2 binding to CD25.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Functional Studies, Immunofluorescence
Species Reactivity
Human
Research Areas
Cardiovascular, Immuno Oncology, Immunology, AKT Signaling, B Cell Markers, Cytokine Signaling, Hematopoietic Stem Cells, Immune checkpoint, Mast Cell Marker, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Membrane
Gene Name
IL2RA
Positive Control
PHA-stimulated human lymphocytes. Human lymph nodes and tonsils.
Immunogen
Stimulated human leukocytes
Alternate Names
Interleukin-2 receptor subunit alpha, TAC antigen, p55, IL2 Receptor alpha; IL2R alpha chain; IL2R; IL2R subunit alpha IL2RA; Interleukin-2 receptor subunit alpha; Ly43; p55; p55 chain; T Cell Growth Factor Receptor; TAC antigen; TCGFR

Database Links

Entrez Gene ID
3559
SwissProt
P01589

Additional Information

Clone
143-13
Chromosome Location
10p15-p14
Mol. Weight of Antigen
55kDa

Related Antibodies

View All IL2RA Antibodies

Functions

  • Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

Key References

  • Knapp W. et al. Leucocyte typing IV, p. 408- 411 and p. 1080, Oxford University Press, Oxford 1989.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “CD25 / IL2RA (Activated Lymphocyte Marker) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

We hold Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales