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Recombinant CD25 / IL2RA (Activated Lymphocyte Marker) Antibody [IL2RA/4375R]

Additional information

Catalog Number: 3559-RBM9
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
3559-RBM9-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3559-RBM9-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3559-RBM9-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
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  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
IL2RA/4375R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell surface.
Gene Name
IL2RA
Positive Control
Hodgkin's lymphoma|Human lung carcinoma|liver|or thymus.|Tonsil
Immunogen
Recombinant fragment (around aa1-250) corresponding to human IL-2 receptor alpha
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for diagnosis.

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Other Information

Functions
  • Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Key References
  • N.A. Azab, et al. Clinical Immunology. 2008 127(2):151-157.
  • Zhang B, et al. Ann Rheum Dis 2008;67:1037-1040.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3559
SwissProt
P01589
Chromosome Location
10p15.1
Mol. Weight of Antigen
31kDa
Alternate Names
Interleukin-2 receptor subunit alpha, TAC antigen, p55, IL2 Receptor alpha; IL2R alpha chain; IL2R; IL2R subunit alpha IL2RA; Interleukin-2 receptor subunit alpha; Ly43; p55; p55 chain; T Cell Growth Factor Receptor; TAC antigen; TCGFR

Summary

CD25, or interleukin 2 (IL2) receptor alpha (IL2RA), and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (CD25) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble CD25 has been isolated and determined to result from extracellular proteolysis. CD25 (the p55 chain of the interleukin-2 receptor) is a marker of activation for a number of cell types, including T cells, B cells, and macrophages. It also appears to be a reliable immunohistochemical marker for the discrimination of neoplastic from normal/reactive mast cells, with potential as a diagnostic tool in systemic mastocytosis. CD25 is also expressed on hairy cell leukemia.

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