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Home >> Antibodies >> CD16 / FcγReceptor III

CD16 / FcγReceptor III

Mouse Monoclonal Antibody [Clone HuNK2]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified CD16 Mouse Monoclonal Antibody (HuNK2). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

FcRIIIa; FCR-10; FcRIII; FCGRIII; FcR-10; Fc fragment of IgG, low affinity IIIa, receptor for (CD16); neutrophil-specific antigen NA; CD16a; low affinity immunoglobulin gamma Fc region receptor III-A; Fc-gamma RIIIa; immunoglobulin G Fc receptor III; Fc-gamma RIII-alpha; Fc-gamma receptor IIIb (CD16); CD16a antigen; Fc-gamma receptor III-2 (CD 16); FCGR3; Fc gamma receptor III-A; FCG3; low affinity III, receptor for (CD16); Fc-gamma RIII; IGFR3FcgammaRIIIA; CD16FCRIIIA; FCRIII; Fc fragment of IgG, low affinity IIIa, receptor (CD16a)

Positive Control

K-562 or U-937 cells. Lymph nodes and tonsils.

Known Applications & Suggested Dilutions

Func., Flow Cyt, IF         More Details

Functional Studies (Order Ab without BSA & Azide)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Human peripheral blood lymphocytes from a NK-leukemia patient.

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

50-80kDa

Specificity & Comments

It recognizes CD16 (FcgammaRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80kDa. Two similar genes represent CD16, CD16A (FcgammaRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (FcgammaRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Lanier LL et al. Functional properties of a unique subset of cytotoxic CD3+ T lymphocytes that express Fc receptors for IgG (CD16/Leu-11 antigen). J Exp Med 1985, 162(6):2089-2106.
  2. Nagarajan S et al. Ligand binding and phagocytosis by CD16 (Fc gamma receptor III) isoforms. Phagocytic signaling by associated zeta and gamma subunits in Chinese hamster ovary cells. J Biol Chem 1995, 270(43):25762-25770
  3. Cerboni C et al. CD16-mediated activation of phosphatidylinositol-3 kinase (PI-3K) in human NK cells involves tyrosine phosphorylation of Cbl and its association with Grb2, Shc, pp36 and p85 PI-3K subunit. Eur J Immunol 1998, 28(3):1005-1015.
  4. McKenzie SE and Schreiber AD. Biological advances and clinical applications of Fc receptors for IgG. Curr Opin Hematol 1994, 1(1):45-52.
  5. Knapp W. et al. (eds) Leukocyte Typing IV, Oxford University Press, Oxford, 1989.
  6. Braakman E et al. CD16 on human T lymphocytes: expression, function, and specificity for Mouse IgG isotypes. Cell Immunol 1992, 143:97-107.

Bioinformatics

  •  Entrez Gene ID: 2214
  •  Gene Symbol: FCGR3A
  •  SwissProt: P08637
  •  Chromosome Location: 1q23
  •  Unigene: 372679
NBT00928