Home >> Antibodies >> AMACR / p504S (Prostate Cancer Marker)

AMACR / p504S (Prostate Cancer Marker)

Recombinant Rabbit Monoclonal Antibody [Clone AMACR/4572R]

Printable

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Bioinformatics

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with AMACR Recombinant Rabbit Monoclonal Antibody (AMACR/4572R).

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with AMACR Recombinant Rabbit Monoclonal Antibody (AMACR/4572R).

Western Blot Analysis of human kidney tissue lysate using AMACR Recombinant Rabbit Monoclonal Antibody (AMACR/4572R).

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with AMACR Recombinant Rabbit Monoclonal Antibody (AMACR/4572R).

Western Blot Analysis of human kidney tissue lysate using AMACR Recombinant Rabbit Monoclonal Antibody (AMACR/4572R).

Product Details

Synonyms

Alpha-methylacyl-CoA Racemase, CBAS4, Da1-8, Macr1, RACE, RM

Positive Control

PC3 or HEK cells. Human kidney, liver or prostate adenocarcinoma.

Known Applications & Suggested Dilutions

IF, Western Blot, IHC-P More Details

Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human AMACR protein

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

42kDa

Specificity & Comments

This antibody recognizes a protein of 42kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and -oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.