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Home >> Antibodies >> AMACR / p504S (Prostate Cancer Marker)

AMACR / p504S (Prostate Cancer Marker)

Rabbit Polyclonal Antibody

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with AMACR / p504S Rabbit Polyclonal Antibody.

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Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with AMACR / p504S Rabbit Polyclonal Antibody.

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SDS-PAGE Analysis Purified AMACR / p504S Rabbit Polyclonal Antibody.

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Western Blot analysis of Kidney tissue lysate using AMACR Mouse Monoclonal Antibody (Rabbit PAb).

Product Details

Synonyms

Alpha-methylacyl-CoA Racemase, CBAS4, Da1-8, Macr1, RACE, RM

Positive Control

HEK cells. Prostate Adenocarcinoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1:50-1:100)
Immunohistochemistry (Formalin-fixed) (1:50-1:100 for 30-60 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

A synthetic peptide from human AMACR protein

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

42kDa

Specificity & Comments

This antibody recognizes a protein of 54kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and -oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.

Related Products



200ug/ml of Ab Purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Vanguri VK, Woda BA, Jiang Z. Sensitivity of P504S/alpha-methylacyl-CoA racemase (AMACR) immunohistochemistry for the detection of prostate carcinoma on stored needle biopsies. Appl Immunohistochem Mol Morphol. 2006 Sep;14(3):365-8.

Bioinformatics

  •  Entrez Gene ID: 23600
  •  Gene Symbol: AMACR
  •  SwissProt: Q9UHK6
  •  Chromosome Location: 5p13.3
  •  Unigene: 508343
NBT00731