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Home >> Antibodies >> AMACR / p504S (Prostate Cancer Marker)

AMACR / p504S (Prostate Cancer Marker)

Rabbit Monoclonal Antibody [Clone 13H4]

Catalog #

Size

0.1 ml
0.5 ml

Price (USD)

199.00
429

Formulation

Culture Supernatant
Culture Supernatant

Catalog No

Size

0.1 ml
0.5 ml

Formulation

Culture Supernatant
Culture Supernatant

Price (USD)

199.00
429
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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with AMACR / p504S Rabbit Monoclonal Antibody (13H4)

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Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with AMACR / p504S Rabbit Monoclonal Antibody (13H4)

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Western Blot Analysis of Human Kidney tissue lysate using AMACR / p504S Rabbit Monoclonal Antibody (13H4).

Product Details

Synonyms

Alpha-methylacyl-CoA Racemase, CBAS4, Da1-8, Macr1, RACE, RM

Positive Control

HEK cells. Prostate Adenocarcinoma and human kidney tissue.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1:50-1:100)
Immunohistochemistry (Formalin-fixed) (1:50-1:100 for 30-60 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Full length human recombinant AMACR protein

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

42kDa

Specificity & Comments

This antibody recognizes a protein of 54kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and -oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.

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Culture Supernatant with 0.05% Azide.

Key References

  1. Jiang Z et. al. Human Pathology. 2003; 34(8):792
  2. Xu J et. al. Canc Res. 2000; 60:1677
  3. Jiang Z et. al. Am J Surg Pathol. 2001; 25(11):1397

Bioinformatics

  •  Entrez Gene ID: 23600
  •  Gene Symbol: AMACR
  •  SwissProt: Q9UHK6
  •  Chromosome Location: 5p13.3
  •  Unigene: 508343
NBT00664