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Home >> Antibodies >> Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker)

Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker)

Mouse Monoclonal Antibody [Clone rALP/870]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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IHC analysis of formalin-fixed, paraffin-embedded human placenta stained using rALP/870 at 2ug/ml in PBS for 30min RT.

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IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Negative tissue control using rALP/870 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

img

IHC analysis of formalin-fixed, paraffin-embedded human testis. Negative tissue control using rALP/870 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

img

IHC analysis of formalin-fixed, paraffin-embedded human kidney. Negative tissue control using rALP/870 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

img

IHC analysis of formalin-fixed, paraffin-embedded human heart. Negative tissue control using rALP/870 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

img

IHC analysis of formalin-fixed, paraffin-embedded human colon. Negative tissue control using rALP/870 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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SDS-PAGE Analysis Purified Alkaline Phosphatase Recombinant Mouse Monoclonal (rALP/870). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Alkaline phosphatase placental type; Alkaline phosphatase Regan Isozyme; Alp1; ALPP; Germ-cell alkaline phosphatase; nagao Isozyme; PALP; Placental alkaline phosphatase 1; placental heat-stable alkaline phosphatase; PLAP1

Positive Control

JEG-3 or HepG2 cells. Human placenta or seminoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human ALPP protein

Cellular Localization

Cytoplasmic. Cell surface.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

70kDa

Specificity & Comments

Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Wick, MR, et al. 1987; Hum Pathol. 18(9):946-54.

Bioinformatics

  •  Entrez Gene ID: 250
  •  Gene Symbol: ALPP
  •  SwissProt: P05187
  •  Chromosome Location: 2q37.1
  •  Unigene: 284255