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Recombinant Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Antibody [ALPP/2899R]

These antibodies perform exceptionally well in the intended applications and have undergone stringent testing and additional validation to ensure monospecificity.

The MAbMan recommends these antibodies for repeatable and reliable results.

Additional information

Catalog Number: 250-RBM6
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
250-RBM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
250-RBM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
250-RBM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Antibody

Formalin-fixed, paraffin-embedded human placenta stained with PLAP Rabbit Recombinant Monoclonal Antibody (ALPP/2899R).

Recombinant Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Antibody - Image 2

SDS-PAGE Analysis Purified PLAP Rabbit Recombinant Monoclonal Antibody (ALPP/2899R). Confirmation of Integrity and Purity of Antibody.

Recombinant Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Antibody - Image 3

Western Blot Analysis of JEG-3 cell lysate using PLAP Rabbit Recombinant Monoclonal Antibody (ALPP/2899R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
ALPP/2899R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell membrane
Gene Name
ALPP
Positive Control
JEG-3 or HepG2 cells. Placenta or seminoma.
Immunogen
Recombinant full-length human ALPP protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Catalog Number: 100507436-MSM3
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Gene:

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Alkaline phosphatase that can hydrolyze various phosphate compounds.
Key References
  • Wick, MR, et al. 1987;Hum Pathol. 18(9):946-54.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
250
SwissProt
P05187
Chromosome Location
2q37.1
Mol. Weight of Antigen
70kDa
Alternate Names
Alkaline phosphatase, placental type, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, Alkaline phosphatase placental type; Alkaline phosphatase Regan Isozyme; ALP; Alp1; ALPP; Germ-cell alkaline phosphatase; nagao Isozyme; PALP; Placental alkaline phosphatase 1; placental heat-stable alkaline phosphatase; PLAP-1; PLAP1

Summary

Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.

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