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A Western blot, or immunoblotting, is a widely used technique for detecting and analyzing specific proteins within a complex mixture. A Western blot provides information about protein identification, protein size and post-translational modifications, and relative protein expression levels in different samples. Additionally, Western blotting is often used to validate the specificity and performance of antibodies, ensuring that they recognize the intended target [link]. Overall, Western blotting is a versatile and widely used technique in molecular biology and biochemistry, providing information about protein presence, size, and relative abundance, which is essential for a wide range of research applications, including protein characterization, biomarker identification, and the study of protein expression in health and disease.
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