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Recombinant Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker) Antibody [KRT10/7804R]

In Stock
Catalog Number Formulation Size Price
3858-RBM14-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
3858-RBM14-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
3858-RBM14-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker) Antibody

IHC analysis of formalin-fixed, paraffin-embedded human skin. Stained using KRT10/7804R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Cytokeratin 10 Antibody in Western Blot (WB)

Cytokeratin 10 Antibody in Western Blot (WB)

Western blot analysis of Human Skin tissue lysate using Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody (KRT10/7804R).

Cytokeratin 10 Antibody in Western Blot (WB)

Cytokeratin 10 Antibody in Western Blot (WB)

Western blot analysis of Skin tissue lysate of different species using Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody (KRT10/7804R).

KRT10 Antibody in SDS-PAGE

KRT10 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Keratin, type I cytoskeletal 10 Recombinant Rabbit Monoclonal Antibody (KRT10/7804R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This MAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Developmental Biology
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm
Gene Name
KRT10
Positive Control
Human skin.
Immunogen
Recombinant human fragment (around aa 500-600) of human KRT10 protein (exact sequence is proprietary)
Alternate Names
Keratin, type I cytoskeletal 10, Cytokeratin-10, Keratin-10, BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10

Database Links

Entrez Gene ID
3858
SwissProt
P13645

Additional Information

Clone
KRT10/7804R
Chromosome Location
17q21.2
Mol. Weight of Antigen
56.5kDa

Related Antibodies

View All KRT10 Antibodies

Functions

  • Plays a role in the establishment of the epidermal barrier on plantar skin (By similarity). Involved in the maintenance of cell layer development and keratin filament bundles in suprabasal cells of the epithelium (By similarity).
  • (Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization.
  • (Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur.

Key References

  • Barrott JJ et al. Proc Natl Acad Sci U S A108:12752-7 (2011).
  • Reichelt J et al. J Cell Sci110 ( Pt 18):2175-86 (1997).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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