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Recombinant Cytokeratin 10 (Suprabasal Epithelial Marker) Antibody [MSVA-610M]

In Stock
Catalog Number Formulation Size Price
3858-MSM18-P0
Purified Ab with BSA and Azide
20ug
$249.00
3858-MSM18-P1
Purified Ab with BSA and Azide
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
KRT10 Antibody in Numerous Tissues (IHC)

KRT10 Antibody in Numerous Tissues (IHC)

Keratin, type I cytoskeletal 10 Mouse Recombinant Monoclonal Antibody (MSVA-610M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:100-1:200
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

This MAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Autophagy, Developmental Biology
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell surface, Extracellular space, Secreted
Gene Name
KRT10
Positive Control
Skin: All squamous epithelial cells (except the basal cell layer) should show strong cytoplasmic staining, while the basal cell layer remains KRT10 negative or shows only minimal staining.
Immunogen
Recombinant full-lenth human KRT10 protein.
Alternate Names
Keratin, type I cytoskeletal 10, Cytokeratin-10, Keratin-10, BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10

Database Links

Entrez Gene ID
3858
SwissProt
P13645

Additional Information

Clone
MSVA-610M
Chromosome Location
17q21.2
Mol. Weight of Antigen
56.5kDa

Related Antibodies

View All KRT10 Antibodies

Functions

  • Plays a role in the establishment of the epidermal barrier on plantar skin.
  • (Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization.
  • (Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur.

Key References

  • Ivanyi D, et. al. Journal of Pathology, 1989, 159:7-12.
  • Ivanyi D, et. al. Differentiation, 1989, 42(2):124-9.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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