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Recombinant TdT / DNA Nucleotidylexotransferase (Acute Lymphoblastic Leukemia Marker) Antibody [DNTT/4617R]

In Stock
Catalog Number Formulation Size Price
1791-RBM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1791-RBM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1791-RBM7-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Terminal deoxynucleotidyl transferase (TdT) is an unusual deoxynucleotide polymerizing enzyme with a molecular weight of about 58 kDa found normally only in B- and T-cell lymphoblasts/prelymphocytes. TdT generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Rare TdT-positive cells are regularly detected in thymus and bone marrow. Typically, TdT expression in the thymus is very variable from cell to cell since it is rapidly decreased in more mature T-cells. TdT-positive cells may occasionally be found in tonsils, lymph nodes and extranodal lymphoid tissue. Immunohistochemical detection of TdT has value in classification of malignant lymphomas and acute leukemias, particularly for the identification of pre-B and pre-T acute lymphoblastic leukemia/lymphoblastic lymphoma (ALL/LBL).

Product Properties & Targets

Host
Rabbit
Applications
Species Reactivity
Research Areas
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Nucleus
Gene Name
Positive Control
Human thymus. Jurkat cells.
Immunogen
Recombinant fragment (around aa 52-192) of human DNTT protein (exact sequence is proprietary)
Alternate Names
DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Deoxynucleotidyltransferase terminal; DNTT; Nucleosidetriphosphate DNA Deoxynucleotidylexotransferase; TDT; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal deoxyribonucleotidyltransferase

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
DNTT/4617R
Chromosome Location
10q24.1
Mol. Weight of Antigen
58kDa

Functions

  • Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.

Key References

  • Faber J, et al. Arch Pathol Lab Med. 2000 Jan;124(1):92-7.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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