Formalin-fixed, paraffin-embedded human thymus stained with TdT Recombinant Rabbit Monoclonal Antibody (DNTT/4617R).

Formalin-fixed, paraffin-embedded human thymoma stained with TdT Recombinant Rabbit Monoclonal Antibody (DNTT/4617R).

SDS-PAGE Analysis Purified TdT Recombinant Rabbit Monoclonal Antibody (DNTT/4617R). Confirmation of Purity and Integrity of Antibody.

TdT / DNA Nucleotidylexotransferase (Acute Lymphoblastic Leukemia Marker) [DNTT/4617R]

Name: TdT / DNA Nucleotidylexotransferase (Acute Lymphoblastic Leukemia Marker)
SKU: 1791-RBM7
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

Terminal deoxynucleotidyl transferase (TdT) is an unusual deoxynucleotide polymerizing enzyme with a molecular weight of about 58 kDa found normally only in B- and T-cell lymphoblasts/prelymphocytes. TdT generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Rare TdT-positive cells are regularly detected in thymus and bone marrow. Typically, TdT expression in the thymus is very variable from cell to cell since it is rapidly decreased in more mature T-cells. TdT-positive cells may occasionally be found in tonsils, lymph nodes and extranodal lymphoid tissue. Immunohistochemical detection of TdT has value in classification of malignant lymphomas and acute leukemias, particularly for the identification of pre-B and pre-T acute lymphoblastic leukemia/lymphoblastic lymphoma (ALL/LBL).

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Product Properties & Targets

Host

Rabbit

Species Reactivity

Human

Isotype

IgG

Cellular Localization

Nucleus

Gene Name

DNTT

Mol. Weight of Antigen

58kDa

Immunogen

Recombinant fragment (around aa 52-192) of human DNTT protein (exact sequence is proprietary)

Database Links

Entrez Gene ID

1791

Unigene

534206

SwissProt

P04053

Additional Information

Clone

DNTT/4617R

Human Gene Symbol

DNTT

Chromosome Location

10q24.1

Known Applications & Suggested Dilutions

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Faber J, et al. Arch Pathol Lab Med. 2000 Jan;124(1):92-7.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.