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Recombinant TdT / DNA Nucleotidylexotransferase (Acute Lymphoblastic Leukemia Marker) Antibody [MSVA-453R]

In Stock
Catalog Number Formulation Size Price
1791-RBM11-P0
Purified Ab with BSA and Azide
20ug
$324.50
1791-RBM11-P1
Purified Ab with BSA and Azide
100ug
$599.50
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
DNTT Antibody in Numerous Tissues (IHC)

DNTT Antibody in Numerous Tissues (IHC)

DNA nucleotidylexotransferase Rabbit Recombinant Monoclonal Antibody (MSVA-453R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:75-1:150
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

Terminal deoxynucleotidyl transferase (TdT) is an unusual deoxynucleotide polymerizing enzyme with a molecular weight of about 58 kDa found normally only in B- and T-cell lymphoblasts/prelymphocytes. TdT generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Rare TdT-positive cells are regularly detected in thymus and bone marrow. Typically, TdT expression in the thymus is very variable from cell to cell since it is rapidly decreased in more mature T-cells. TdT-positive cells may occasionally be found in tonsils, lymph nodes and extranodal lymphoid tissue. Immunohistochemical detection of TdT has value in classification of malignant lymphomas and acute leukemias, particularly for the identification of pre-B and pre-T acute lymphoblastic leukemia/lymphoblastic lymphoma (ALL/LBL).

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
B Cell Markers, Nuclear Marker
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Nucleus
Gene Name
DNTT
Positive Control
Thymus: At least a moderate nuclear immunostaining should be seen in virtually all cortical lymphocytes of the normal thymus.
Immunogen
Recombinant fragment (around aa 52-192) of human DNTT protein (exact sequence is proprietary)
Alternate Names
DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Deoxynucleotidyltransferase terminal; DNTT; Nucleosidetriphosphate DNA Deoxynucleotidylexotransferase; TDT; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal deoxyribonucleotidyltransferase

Database Links

Entrez Gene ID
1791
SwissProt
P04053

Additional Information

Clone
MSVA-453R
Chromosome Location
10q24.1
Mol. Weight of Antigen
58kDa

Related Antibodies

View All DNTT Antibodies

Functions

  • Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.

Key References

  • Faber J, et al. Arch Pathol Lab Med. 2000 Jan;124(1):92-7.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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