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Formalin-fixed, paraffin-embedded human Thymus stained with TdTMouse Monoclonal Antibody (DNTT/1453).
Formalin-fixed, paraffin-embedded human Thymus stained with TdTMouse Monoclonal Antibody (DNTT/1453).
Formalin-fixed, paraffin-embedded human Thymus stained with TdTMouse Monoclonal Antibody (DNTT/1453).
SDS-PAGE Analysis of Purified TdT Mouse Monoclonal Antibody (DNTT/1453). Confirmation of Purity and Integrity of Antibody.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
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Terminal deoxynucleotidyltransferase (TdT) is a DNA polymerase which catalyzes the addition of deoxyribonucleotides onto the 3′-hydroxyl end of DNA primers without template direction. The enzyme thus provides a unique method for the labeling of the 3′ termini of DNA. The human TdT gene maps to chromosome 10q24.1 and encodes a 510 amino acid protein. Human TdT is synthesized as a single chain peptide that elicits a minor preference for incorporation of deoxyribonucleotides over ribonucleotides forming DNA strands. TdT is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells.
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