Formalin-fixed, paraffin-embedded human Thymus stained with TdTMouse Monoclonal Antibody (DNTT/1453).

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TdT / DNA Nucleotidylexotransferase (Acute Lymphoblastic Leukemia Marker) [DNTT/1453]

Name: TdT / DNA Nucleotidylexotransferase (Acute Lymphoblastic Leukemia Marker)
SKU: 1791-MSM5
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

Terminal deoxynucleotidyltransferase (TdT) is a DNA polymerase which catalyzes the addition of deoxyribonucleotides onto the 3'-hydroxyl end of DNA primers without template direction. The enzyme thus provides a unique method for the labeling of the 3' termini of DNA. The human TdT gene maps to chromosome 10q24.1 and encodes a 510 amino acid protein. Human TdT is synthesized as a single chain peptide that elicits a minor preference for incorporation of deoxyribonucleotides over ribonucleotides forming DNA strands. TdT is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells.

Product Properties & Targets

Host

Mouse

Species Reactivity

Human

Isotype

IgG1

Cellular Localization

Nucleus

Light Chain

kappa

Gene Name

DNTT

Positive Control

Human thymus. Jurkatcells.

Immunogen

Recombinant fragment (around aa 52-192) of human DNTT protein (exact sequence is proprietary)

Alternate Names

DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Deoxynucleotidyltransferase terminal; DNA Nucleotidylexotransferase; DNTT; Nucleosidetriphosphate DNA Deoxynucleotidylexotransferase; TDT; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal deoxyribonucleotidyltransferase; Terminal transferase

Database Links

Entrez Gene ID

1791

Unigene

534206

SwissProt

P04053

Additional Information

Clone

DNTT/1453

Human Gene Symbol

DNTT

Chromosome Location

10q24.1

Mol. Weight of Antigen

58kDa

Functions

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.

Known Applications & Suggested Dilutions

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Faber J, et al. Arch Pathol Lab Med. 2000 Jan;124(1):92-7.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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