Formalin-fixed, paraffin-embedded human thymus stained with TdT Recombinant Mouse Monoclonal Antibody (rDNTT/6909).

SDS-PAGE Analysis Purified TdT Recombinant Mouse Monoclonal Antibody (rDNTT/6909). Confirmation of Purity and Integrity of Antibody.

Printable Datasheet

Recombinant TdT / DNA Nucleotidylexotransferase (Acute Lymphoblastic Leukemia Marker) [rDNTT/6909]

Name: TdT / DNA Nucleotidylexotransferase (Acute Lymphoblastic Leukemia Marker)
SKU: 1791-MSM9
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies Recombinant Mouse Monoclonals Recombinants

Summary

Terminal deoxynucleotidyl transferase (TdT) is an unusual deoxynucleotide polymerizing enzyme with a molecular weight of about 58 kDa found normally only in B- and T-cell lymphoblasts/prelymphocytes. TdT generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Rare TdT-positive cells are regularly detected in thymus and bone marrow. Typically, TdT expression in the thymus is very variable from cell to cell since it is rapidly decreased in more mature T-cells. TdT-positive cells may occasionally be found in tonsils, lymph nodes and extranodal lymphoid tissue. Immunohistochemical detection of TdT has value in classification of malignant lymphomas and acute leukemias, particularly for the identification of pre-B and pre-T acute lymphoblastic leukemia/lymphoblastic lymphoma (ALL/LBL).

Product Properties & Targets

Host

Mouse

Applications

ELISA, Immunohistochemistry

Species Reactivity

Human

Isotype

IgG1

Cellular Localization

Nucleus

Light Chain

Kappa

Gene Name

DNTT

Positive Control

Human thymus. Jurkat cells.

Immunogen

Recombinant fragment (around aa1-100) of human DNTT protein (exact sequence is proprietary)

Alternate Names

DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Deoxynucleotidyltransferase terminal; DNTT; Nucleosidetriphosphate DNA Deoxynucleotidylexotransferase; TDT; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal deoxyribonucleotidyltransferase

Database Links

Entrez Gene ID

1791

Unigene

534206

SwissProt

P04053

Additional Information

Clone

rDNTT/6909

Human Gene Symbol

DNTT

Chromosome Location

10q24.1

Mol. Weight of Antigen

58kDa

Functions

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.

Known Applications & Suggested Dilutions

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Chang LM, Bollum FJ. CRC Crit Rev Biochem. 1986;21(1):27-52.
Kang LC, Dunphy CH. Arch Pathol Lab Med. 2006;130(2):153-7.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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