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Recombinant SPARC / Osteonectin / O-Nectin Antibody [r15G12]

In Stock
Catalog Number Formulation Size Price
6678-MSM20-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
6678-MSM20-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
6678-MSM20-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Osteonectin Antibody in Immunohistochemistry (IHC (P))

Osteonectin Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human testis stained with SPARC / Osteonectin Recombinant Mouse Monoclonal Antibody (r15G12). Inset: PBS instead of primary antibody; secondary only negative control.

Osteonectin Antibody in Western Blot (WB)

Osteonectin Antibody in Western Blot (WB)

Western blot analysis of A-375 cell lysate using SPARC / Osteonectin Recombinant Mouse Monoclonal Antibody (r15G12).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Osteonectin (ON), also known as BM-40 or SPARC (secreted protein, acidic and rich in cysteine) is a multifunctional glycoprotein (32.5 kD) involved with tissue mineralization as well as extracellular matrix modelling. It is the most abundant glycoprotein secreted by human osteoblasts in developing bone and odontoblasts of developing teeth. ON mRNA and protein have been detected in non-mineralized tissues such as steroid-producing cells of the adrenal glands, suprabasal layers of the epidermis, glomeruli in the kidney, bronchi of the lung, megakaryocytes and large vessels. This organ-specific distribution in non-mineralized tissues suggests a role during human development.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Endothelial Cell Marker, Mesenchymal Stem Cell Differentiation, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Basement membrane, Extracellular matrix, Extracellular space, Secreted
Gene Name
SPARC
Positive Control
Human placenta, lymph node or spleen. JEG3, A-375 or A549 cell lysates.
Immunogen
Prokaryotic recombinant protein corresponding to a portion of the C-terminus of the human osteonectin molecule
Alternate Names
SPARC, Basement-membrane protein 40, Osteonectin, Secreted protein acidic and rich in cysteine, Basement membrane protein 40; BM40; Cysteine rich protein; OI17; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted protein acidic and cysteine rich; Secreted protein acidic and rich in cysteine (SPARC)

Database Links

Entrez Gene ID
6678
SwissProt
P09486

Additional Information

Clone
r15G12
Chromosome Location
5q33.1
Mol. Weight of Antigen
38kDa

Related Antibodies

View All SPARC Antibodies

Functions

  • Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Key References

  • Galv n, J.A., et al. 2019. Immunohistochemical analysis of the expression of cancer-associated fibroblast markers in esophageal cancer with and without neoadjuvant therapy. Virchows Arch. E-published.
  • Yan, L., et al. 2017. Insulin-like growth factor-1 promotes the proliferation and odontoblastic differentiation of human dental pulp cells under high glucose conditions. Int. J. Mol. Med. 40: 1253-1260.
  • Yiu G K, Chan W Y, Ng S-W, et al. American Journal of Pathology. 159 (2): 609–622 (2001).
  • Le Bail B, Faouzi S, Boussarie L, et al. Journal of Pathology. 189: 46–52 (1999).
  • Massi D, Franchi A, Borgognoni L, et al. Human Pathology. 30 (3): 339–344 (1999).
  • Rempel S A, Ge S and Gutiérrez J A. Clinical Cancer Research. 5: 237–241 (1999).
  • Hunzelmann N, Hafner M, Anders S, et al. Journal of Investigative Dermatology. 110: 122–126 (1998).
  • Fanburg J C, Rosenberg A E, Weaver D L, et al. Journal of Clinical Pathology. 108: 464–473 (1997).
  • Frizell E, Liu S L, Abraham A, et al. Hepatology. 21 (3): 847–854 (1995).
  • Mundlos S, Schwahn B, Reichert T, et al. The Journal of Histochemistry and Cytochemistry. 40 (2): 283–291 (1992).
  • Schulz A, Jundt G, Berghäuser K-H, et al. American Journal of Pathology. 132 (2): 233–238 (1988).
  • Lindner, J L et al. “Expression of secreted protein acidic and rich in cysteine (SPARC) in breast cancer and response to neoadjuvant chemotherapy.” Annals of oncology : official journal of the European Society for Medical Oncology vol. 26,1 (2015): 95-100. doi:10.1093/annonc/mdu487
  • Hartley, Christopher et al. “Increased SPARC expression is associated with neoadjuvant therapy in resectable pancreatic ductal adenocarcinoma.” Practical laboratory medicine vol. 21 e00171. 29 May. 2020, doi:10.1016/j.plabm.2020.e00171

PubMed Links

Storage & Stability

Antibody with azide store at 2 to 8 °C. Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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