SPARC / Osteonectin Antibody [OSTN/3761]

These antibodies are validated on HuProt™ Human Protein Microarrays containing over 21,000 full-length human proteins, including the specific target of interest. LEARN MORE

Additional information

Catalog Number: 6678-MSM11
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

In Stock
Catalog Number Formulation Size Price
6678-MSM11-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
6678-MSM11-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6678-MSM11-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
  • IHC
  • WB

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Host
Mouse
Species Reactivity
Clone
OSTN/3761
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Basement membrane, Extracellular matrix, Extracellular space, Secreted
Gene Name
Positive Control
Human placenta, lymph node or spleen. JEG3, A-375 or A549 cell lysates.
Immunogen
Recombinant fragment (around aa1-200) of human SPARC protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide store at 2 to 8 °C. Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

Related Antibodies

Other Information

Functions
Key References
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
SwissProt
Chromosome Location
5q33.1
Mol. Weight of Antigen
38kDa
Alternate Names
SPARC, Basement-membrane protein 40, Osteonectin, Secreted protein acidic and rich in cysteine, Basement membrane protein 40; BM40; Cysteine rich protein; OI17; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted protein acidic and cysteine rich; Secreted protein acidic and rich in cysteine (SPARC)

Summary

SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.

Worry-Free Guarantee

Should a NeoBiotechnologies antibody not meet the experimental expectations outlined on our website or datasheet, please contact our Technical Support. We unconditionally guarantee our product.

Need Technical Help?

Our team of scientists are ready to help you with expert recommendations or technical assistance related to applications or product use.