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Recombinant p27Kip1 (Mitotic Inhibitor / Suppressor Protein) Antibody [r1B4]

In Stock
Catalog Number Formulation Size Price
1027-MSM12-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
1027-MSM12-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1027-MSM12-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
p27Kip1 Antibody in Immunohistochemistry (IHC (P))

p27Kip1 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human placenta stained with p27Kip1 Recombinant Mouse Monoclonal Antibody (r1B4). Inset: PBS instead of primary antibody; secondary only negative control.

p27 Antibody in Western Blot (WB)

p27 Antibody in Western Blot (WB)

Western blot analysis of SK-BR-3 cell lysate using p27 Recombinant Mouse Monoclonal Antibody (r1B4).

p27Kip1 Antibody in SDS-PAGE

p27Kip1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified p27 Recombinant Mouse Monoclonal Antibody (r1B4). Confirmation of Purity and Integrity of Antibody.

p27 Antibody in Western Blot (WB)

p27 Antibody in Western Blot (WB)

Western blot analysis of Human Brain and Human Spleen tissue lysates using p27 Recombinant Mouse Monoclonal Antibody (r1B4).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

p27 protein, also known as kinase inhibitory protein 1 (Kip1), is a polypeptide which binds to cyclin E/cdk2 complexes (but not to cdk2 alone) and is detected in purified extracts of growth-arrested cells. p27 protein constrains cell proliferation by setting the threshold level of cyclin E necessary to activate cdk2. The 27kD protein is also present in proliferating cells but only in a sequestered form when it is unavailable to interact with cyclin E/cdk2 complexes. It is likely that cyclin D complexed with catalytically inactive cdk4 is sufficient to sequester p27 and titrate its function. The presence of bound p27 in proliferating cells suggests that its role may not be restricted to inducing cell cycle arrest but to also set the cyclin E threshold for execution of the G1 to S phase transition during each mitotic cycle.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cancer, Cardiovascular, Immunology, AKT Signaling, Cytokine Signaling, Infectious Disease, Lung Cancer, Nuclear Marker, Ovarian Cancer, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Endosome, Nucleus
Gene Name
CDKN1B
Positive Control
ZR75, MCF7, MDA-MB-231 or SK-BR-3 cells. Human placenta,Brain, tonsil or spleen.
Immunogen
Prokaryotic recombinant antigen corresponding to the full length p27 molecule
Alternate Names
Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, KIP1, MEN1B, MEN4

Database Links

Entrez Gene ID
1027
SwissProt
P46527

Additional Information

Clone
r1B4
Chromosome Location
12p13.1
Mol. Weight of Antigen
25-26kDa
Expression System
CHO Cells

Related Antibodies

View All CDKN1B Antibodies

Functions

  • Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Key References

  • Fredersdorf S, et. al. Proc Natl Acad Sci 1997;94:6380-5.
  • Wong S C C, Chan J K C, Lee K C, et al. Journal of Pathology. 194: 35–42 (2001).
  • Fernández-Figueras M T, Puig L, Penín R M, et al. Journal of Pathology. 191: 387–393 (2000).
  • Polyak K, Kato J-Y, Solomon M J, et al. Genes and Development. 8: 9–22 (1994).
  • Toyoshima H and Hunter T. Cell. 78: 67–74 (1994).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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