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Recombinant Napsin A (Lung Adenocarcinoma Marker) Antibody [NAPSA/1865R]

In Stock
Catalog Number Formulation Size Price
9476-RBM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
9476-RBM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
9476-RBM5-P1ABX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Napsin A (Lung Adenocarcinoma Marker) Antibody

Formalin-fixed, paraffin-embedded human Lung Adenocarcinoma stained with Napsin A Rabbit Recombinant Monoclonal Antibody (NAPSA/1865R).

Napsin-A Antibody in Western blot (WB)

Napsin-A Antibody in Western blot (WB)

Western Blot Analysis of Human Kidney lysate using Napsin-A Rabbit Recombinant Monoclonal Antibody (NAPSA/1865R).

Napsin A Antibody in Western Blot (WB)

Napsin A Antibody in Western Blot (WB)

Western Blot Analysis of Human Lung, Human Kidney, K-562, HEK293, and A549 lysates using Napsin A Recombinant Rabbit Monoclonal Antibody (NAPSA/1865R).

Napsin A Antibody in Western Blot (WB)

Napsin A Antibody in Western Blot (WB)

Western blot analysis of Human Kidney, Mouse Kidney, Rat Kidney, Hamster Kidney, Guinea pig Kidney, Rabbit Kidney, Human Lung, Mouse Lung, Rat Lung, Hamster Lung, Guinea pig Lung, and Rabbit Lung tissue lysates using Napsin A Recombinant Rabbit Monoclonal Antibody (NAPSA/1865R).

Napsin A Antibody in Western Blot (WB)

Napsin A Antibody in Western Blot (WB)

Western Blot Analysis of Human Brain, Human Heart, Human Intestine, Human Kidney, Human Liver, Human Lung, Human Ovary, Human Pancreas, Human Prostrate, Human Skeletal Muscle, Human Skin, Human Spleen, Human Stomach, and Human Testis tissue lysates using Napsin A Recombinant Rabbit Monoclonal Antibody (NAPSA/1865R).

NAPSA Antibody in SDS-PAGE

NAPSA Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Napsin-A Recombinant Rabbit Monoclonal Antibody (NAPSA/1865R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human, Rabbit, Rat
Research Areas
Dendritic Cell Marker
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Secreted
Gene Name
NAPSA
Positive Control
Lung adenocarcinoma, Lung, Kidney, K-562, HEK293, A549, Human Brain, Human Heart, Human Intestine, Human Kidney, Human Liver, Human Lung, Human Ovary, Human Pancreas, Human Prostrate, Human Skeletal Muscle, Human Skin, Human Spleen, Human Stomach, Human Testis
Immunogen
Recombinant human Napsin-A protein fragment (aa189-299) (exact sequence is proprietary)
Alternate Names
Napsin-A, Aspartyl protease 4, Napsin-1, TA01/TA02, ASP4, Aspartyl protease 4, KAP, Kidney derived aspartic protease like protein (Kdap), NAP1, NAPA, Napsa, napsin A aspartic peptidase, Pronapsin A, SNAPA

Database Links

Entrez Gene ID
9476
SwissProt
O96009

Additional Information

Clone
NAPSA/1865R
Chromosome Location
19q13.33
Mol. Weight of Antigen
37kDa

Related Antibodies

View All NAPSA Antibodies

Functions

  • May be involved in processing of pneumocyte surfactant precursors.

Key References

  • Ordonez, NG 2012 Adv Anat Pathol 19: 66-73
  • Bishop, JA et. al. Hum Pathol 41: 20-25
  • Ye, J et. al. Appl Immunhistochem Mol Morphol 19: 313-317

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of recombinant MAb Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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