Free Shipping in the U.S. for orders over $1000.  Shop Now>>

Printable Datasheet

Recombinant Napsin A (Lung Adenocarcinoma Marker) Antibody [MSVA-112R]

In Stock
Catalog Number Formulation Size Price
9476-RBM20-P0
Purified Ab with BSA and Azide
20ug
$324.50
9476-RBM20-P1
Purified Ab with BSA and Azide
100ug
$599.50
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
NAPSA Antibody in Numerous Tissues (IHC)

NAPSA Antibody in Numerous Tissues (IHC)

Napsin-A Rabbit Recombinant Monoclonal Antibody (MSVA-112R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:100-1:200
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Dendritic Cell Marker
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Secreted
Gene Name
NAPSA
Positive Control
Kidney: an at least moderate granular cytoplasmic staining should be seen in proximal tubule cells
Immunogen
Recombinant human Napsin-A protein fragment (aa189-299) (exact sequence is proprietary)
Alternate Names
Napsin-A, Aspartyl protease 4, Napsin-1, TA01/TA02, ASP4, Aspartyl protease 4, KAP, Kidney derived aspartic protease like protein (Kdap), NAP1, NAPA, Napsa, napsin A aspartic peptidase, Pronapsin A, SNAPA

Database Links

Entrez Gene ID
9476
SwissProt
O96009

Additional Information

Clone
MSVA-112R
Chromosome Location
19q13.33
Mol. Weight of Antigen
37kDa

Related Antibodies

View All NAPSA Antibodies

Functions

  • May be involved in processing of pneumocyte surfactant precursors.

Key References

  • Ordonez, NG 2012 Adv Anat Pathol 19: 66-73
  • Bishop, JA et. al. Hum Pathol 41: 20-25
  • Ye, J et. al. Appl Immunhistochem Mol Morphol 19: 313-317

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales