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Recombinant Napsin A (Lung Adenocarcinoma Marker) Antibody [NAPSA/7165R]

Additional information

Catalog Number: 9476-RBM18
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
9476-RBM18-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
9476-RBM18-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
9476-RBM18-P1ABX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Napsin A (Lung Adenocarcinoma Marker) Antibody

IHC analysis of formalin-fixed, paraffin-embedded human lung adenocarcinoma. NAPSA/7165R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant Napsin A (Lung Adenocarcinoma Marker) Antibody - Image 2

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using NAPSA/7165R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Napsin A Antibody in Western Blot (WB)

Napsin A Antibody in Western Blot (WB)

Western Blot Analysis of Human Brain, Human Heart, Human Intestine, Human Kidney, Human Liver, Human Lung, Human Ovary, Human Pancreas, Human Prostrate, Human Skeletal Muscle, Human Skin, Human Spleen, Human Stomach, and Human Testis tissue lysates using Napsin A Recombinant Rabbit Monoclonal Antibody (NAPSA/7165R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
NAPSA/7165R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm.
Gene Name
NAPSA
Positive Control
Human lung, Human Kidney
Immunogen
Recombinant fragment (aa50-150) of human Napsin A protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • May be involved in processing of pneumocyte surfactant precursors.
Key References
  • Bishop, JA et. al. Hum Pathol 41: 20-25
  • Ordonez, NG 2012 Adv Anat Pathol 19: 66-73
  • Ye, J et. al.Appl Immunhistochem Mol Morphol 19: 313-317
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
9476
SwissProt
O96009
Chromosome Location
19q13.33
Mol. Weight of Antigen
37kDa
Alternate Names
Napsin-A, Aspartyl protease 4, Napsin-1, TA01/TA02, ASP4, Aspartyl protease 4, KAP, Kidney derived aspartic protease like protein (Kdap), NAP1, NAPA, Napsa, napsin A aspartic peptidase, Pronapsin A, SNAPA

Summary

Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B. �There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

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