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Recombinant MGMT (Marker of Suppressor of Mutation and Carcinogenesis) Antibody [MGMT/8364R]

In Stock
Catalog Number Formulation Size Price
4255-RBM11-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4255-RBM11-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4255-RBM11-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant MGMT (Marker of Suppressor of Mutation and Carcinogenesis) Antibody

Formalin-fixed, paraffin-embedded human tonsil stained with MGMT Mouse Monoclonal Antibody (MGMT/8364R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Recombinant MGMT (Marker of Suppressor of Mutation and Carcinogenesis) Antibody - Image 2

Formalin-fixed, paraffin-embedded human prostate stained with MGMT Mouse Monoclonal Antibody (MGMT/8364R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Recombinant MGMT (Marker of Suppressor of Mutation and Carcinogenesis) Antibody - Image 3

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using MGMT/8364R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Recombinant MGMT (Marker of Suppressor of Mutation and Carcinogenesis) Antibody - Image 4

SDS-PAGE Analysis of Purified MGMT Mouse Monoclonal Antibody (MGMT/8364R). Confirmation of Purity and Integrity of Antibody.

MGMT Antibody in Western Blot (WB)

MGMT Antibody in Western Blot (WB)

Western Blot Analysis of MCF-7, LNCaP, Hep G2 and human liver lysates using MGMT Recombinant Rabbit Monoclonal Antibody (MGMT/8364R).

MGMT Antibody in Western Blot (WB)

MGMT Antibody in Western Blot (WB)

Western Blot Analysis of Human Liver, Human Pancreas, Raji and MCF7 lysates using MGMT Recombinant Rabbit Monoclonal Antibody (MGMT/8364R).

MGMT Antibody in Western Blot (WB)

MGMT Antibody in Western Blot (WB)

Western Blot Analysis of Brain and Pancreas lysates of different lysates using MGMT Recombinant Rabbit Monoclonal Antibody (MGMT/8364R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal crossâ � �links at the O6â � � alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6â � �position of guanine in DNA (prior to crossâ � �link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNAâ � �damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Nucleus
Gene Name
MGMT
Positive Control
Jurkat, MCF-7, LNCaP, Hep G2 and human liver. Human tonsil or stomach (IHC).
Immunogen
Recombinant fragment (around aa1-100) of human MGMT (exact sequence is proprietary)
Alternate Names
Methylated-DNA | protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase, 6 O methylguanine DNA methyltransferase; Methylated DNA protein cysteine methyltransferase; O 6 methylguanine DNA alkyltransferase; O 6 methylguanine DNA methyltransferase

Database Links

Entrez Gene ID
4255
SwissProt
P16455

Additional Information

Clone
MGMT/8364R
Chromosome Location
10q26.3
Mol. Weight of Antigen
21.646 kDa

Related Antibodies

View All MGMT Antibodies

Functions

  • Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Key References

  • D Incalci, M., et al. 1988. Importance of the DNA repair enzyme O6-alkylguanine alkyltransferase (AT) in cancer chemotherapy. Cancer Treat. Rev.15: 279-292.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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