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Macrophage & Histiocytoma Marker Antibody [D11]

Additional information

Catalog Number: MSM2-348
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
MSM2-348-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
MSM2-348-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
MSM2-348-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Macrophage & Histiocytoma Marker Antibody

SDS-PAGE Analysis of Purified Macrophage Monoclonal Antibody (D11). Confirmation of Purity and Integrity of Antibody.

Macrophage & Histiocytoma Marker Antibody - Image 2

Formalin-fixed, paraffin-embedded human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).

Macrophage & Histiocytoma Marker Antibody - Image 3

Formalin-fixed, paraffin-embedded human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
D11
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
N/A
Gene Name
N/A
Positive Control
Liver or histiocytoma.
Immunogen
Membrane preparation from human hepatocytes
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% azide. Also available WITHOUT azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • N/A
Key References
  • Rudinskaya TD, Poltoranina VS, Baranov VN, Petrovichev NN, Voytenkov BO, Tretyakov LO. D11, a novel monoclonal antibody specific for human mature macrophages and peripheral blood monocytes. Immunol Lett. 1992 Jun;33(1):1-7.
  • Frenkel MA, Tupitsyn NN, Rudinskaia TD, Poltoranina VS, Lebedeva NB, Volkova MA, Kaletin GI. [Use of a new antimacrophage monoclonal antibody D11 in diagnosis of hemoblastosis]. [Article in Russian] Gematol Transfuziol. 1995 Jul-Aug;40(4):13-6.
  • Tupitsyn NN, et al. Reactivity of anti-macrophage monoclonal antibody D11 in human leukemia and malignant lymphoma. Int J Cancer. 1996;68(2):160-3.
  • Petrovichev NN, et al. Antimacrophage monoclonal antibody D11 in the diagnosis of tumors of histiocytic origin. Acta Cytol. 1997;41(2):357-63. 2.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
Not Known
SwissProt
N/A
Mol. Weight of Antigen
125kDa -135kDa
Alternate Names
N/A, N/A, Not Known

Summary

In Western blotting, it detects an antigen of 125kDa in human liver and 135kDa in tumors of histiocytic origin. Comparative study of this MAb and a standard CD68 MAb showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This MAb shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This MAb does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.

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