Formalin-fixed, paraffin-embedded human Placenta stained with hCG alpha Monoclonal Antibody (SPM552).

Formalin-fixed, paraffin-embedded human Placenta stained with hCG alpha Monoclonal Antibody (SPM552).

Printable Datasheet

HCG-alpha [SPM552]

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Summary

This MAb reacts with a protein of ~13kDa, identified as alpha sub-unit of HCG. HCG is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the subunit is identical to that of thyroid stimulating hormone (TSH) follicle stimulating hormone (FSH), and luteinizing hormone (LH).

Product Properties & Targets

Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Isotype
IgG1
Cellular Localization
Secreted
Light Chain
Kappa
Gene Name
CGA
Positive Control
Placenta.
Immunogen
Recombinant hCG alpha protein
Alternate Names
Glycoprotein hormones alpha chain, Anterior pituitary glycoprotein hormones common subunit alpha, Choriogonadotropin alpha chain, Chorionic gonadotrophin subunit alpha, Follicle-stimulating hormone alpha chain, Follitropin alpha chain, Luteinizing hormone alpha chain, Lutropin alpha chain, Thyroid-stimulating hormone alpha chain, Thyrotropin alpha chain, CG-alpha; CGA; Chorionic Gonadotrophin Alpha; Follicle Stimulating Hormone Alpha; Follitropin Alpha; FSH-alpha; FSHA; GPH Alpha; GPHA1; LHA; LH-alpha; Luteinizing Hormone Alpha; Lutropin Alpha; Thyroid Stimulating Hormone Alpha; Thyrotropin Alpha; TSHA

Database Links

Entrez Gene ID
1081
Unigene
119689
SwissProt
P01215

Additional Information

Clone
SPM552
Human Gene Symbol
CGA
Chromosome Location
6q12-q21
Mol. Weight of Antigen
~13kDa

Functions

  • Shared alpha chain of the active heterodimeric glycoprotein hormones thyrotropin/thyroid stimulating hormone/TSH, lutropin/luteinizing hormone/LH, follitropin/follicle stimulating hormone/FSH and choriogonadotropin/CG. These hormones bind specific receptors on target cells that in turn activate downstream signaling pathways.

Known Applications & Suggested Dilutions

  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
  • Optimal dilution for a specific application should be determined.

Key References

  • McDonald EA et. al. Endocrinology 150:4358-65 (2009).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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