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Recombinant HCG-alpha (Pregnancy & Choriocarcinoma Marker) Antibody [HCGa/2728R]

In Stock
Catalog Number Formulation Size Price
1081-RBM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1081-RBM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1081-RBM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human placenta stained with HCG-alpha Rabbit Recombinant Monoclonal Antibody (HCGa/2728R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human placenta stained with HCG-alpha Rabbit Recombinant Monoclonal Antibody (HCGa/2728R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human placenta stained with HCG-alpha Rabbit Recombinant Monoclonal Antibody (HCGa/2728R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human placenta stained with HCG-alpha Rabbit Recombinant Monoclonal Antibody (HCGa/2728R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Human chorionic gonadotropin antibody (hCG) is a glycoprotein hormone synthesized in syncytiotrophoblastic cells of placenta and in certain trophoblastic tumors. The hormone-specific alpha chains have molecular weights of 13 kDa. HCG is found in moles and choriocarcinoma, chorionic components of germ cell tumors, and syncytiotrophoblast like cells in seminoma/dysgerminoma and embryonal carcinoma. In diagnostic pathology, hCG is a useful marker for classification of germ cell tumors, identification of extragonadal germ cell tumors.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Secreted
Gene Name
CGA
Positive Control
Human placenta.
Immunogen
Recombinant fragment (around aa1-100) of human hCG alpha protein (exact sequence is proprietary)
Alternate Names
Glycoprotein hormones alpha chain, Anterior pituitary glycoprotein hormones common subunit alpha, Choriogonadotropin alpha chain, Chorionic gonadotrophin subunit alpha, Follicle-stimulating hormone alpha chain, Follitropin alpha chain, Luteinizing hormone alpha chain, Lutropin alpha chain, Thyroid-stimulating hormone alpha chain, Thyrotropin alpha chain, CG-alpha; CGA; Chorionic Gonadotrophin Alpha; Follicle Stimulating Hormone Alpha; Follitropin Alpha; FSH-alpha; FSHA; GPH Alpha; GPHA1; LHA; LH-alpha; Luteinizing Hormone Alpha; Lutropin Alpha; Thyroid Stimulating Hormone Alpha; Thyrotropin Alpha; TSHA

Database Links

Entrez Gene ID
1081
SwissProt
P01215

Additional Information

Clone
HCGa/2728R
Chromosome Location
6q12-q21
Mol. Weight of Antigen
~13kDa

Related Antibodies

View All CGA Antibodies

Functions

  • Shared alpha chain of the active heterodimeric glycoprotein hormones thyrotropin/thyroid stimulating hormone/TSH, lutropin/luteinizing hormone/LH, follitropin/follicle stimulating hormone/FSH and choriogonadotropin/CG. These hormones bind specific receptors on target cells that in turn activate downstream signaling pathways.

Key References

  • Cocquebert M et. al. Am J Physiol Endocrinol Metab. 2012;303(8):E950-8.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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