Formalin-fixed, paraffin-embedded human Pancreas stained with Ferritin, Light Chain Recombinant Mouse Monoclonal Antibody (rFTL/1388).

SDS-PAGE Analysis Purified Ferritin LC Recombinant Mouse Monoclonal Antibody (rFTL/1388). Confirmation of Integrity and Purity of Antibody

Western Blot Analysis of human Kidney tissue lysate using Ferritin LC Recombinant Mouse Monoclonal Antibody (rFTL/1388).

Ferritin, Light Chain (Node-Negative Breast Tumor Prognostic Marker) [rFTL/1388]

Name: Ferritin, Light Chain (Node-Negative Breast Tumor Prognostic Marker)
SKU: 2512-MSM7
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies Recombinant Mouse Monoclonals Recombinants

Summary

Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.

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Product Properties & Targets

Host

Mouse

Species Reactivity

Human

Isotype

IgG1

Gene Name

FTL

Mol. Weight of Antigen

19-25kDa

Immunogen

Recombinant fragment (around aa 38-165) of human FTL protein (exact sequence is proprietary)

Database Links

Entrez Gene ID

2512

Unigene

433670

SwissProt

P02792

Additional Information

Clone

rFTL/1388

Human Gene Symbol

FTL

Chromosome Location

19q13.33

Known Applications & Suggested Dilutions

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Key References

Jezequel P, et. al. Validation of tumor-associated macrophage ferritin light chain as a prognostic biomarker in node-negative breast cancer tumors: A multicentric 2004 national PHRC study Int. J Cancer. 2012;131(2):426-37

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.