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Recombinant Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker) Antibody [KRT20/3224R]

These antibodies perform exceptionally well in the intended applications and have undergone stringent testing and additional validation to ensure monospecificity.

The MAbMan recommends these antibodies for repeatable and reliable results.

Additional information

Catalog Number: 54474-RBM6
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
54474-RBM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
54474-RBM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
54474-RBM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Cytokeratin 20 Antibody in Western Blot (WB)

Cytokeratin 20 Antibody in Western Blot (WB)

Western Blot Analysis of HT-29 cell lysate using Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody (KRT20/3224R).

Cytokeratin-20 Antibody in SDS-PAGE

Cytokeratin-20 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified KRT20 Recombinant Rabbit Monoclonal Antibody (KRT20/3224R). Confirmation of Purity and Integrity of Antibody.

  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Western Blot
Species Reactivity
Human
Clone
KRT20/3224R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm
Gene Name
KRT20
Positive Control
HT-29 cell lysate.
Immunogen
Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Additional information

Catalog Number: 100507436-MSM3
Reactivity:

Gene:

Host:

Flow cytometric analysis of PFA-fixed HeLa cells. MEF2B Mouse Monoclonal Antibody (PCRP-MEF2B-2F9) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
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Additional information

Catalog Number: 100271849-MSM1
Reactivity:

Gene:

Host:

Flow cytometric analysis of PFA-fixed HeLa cells. HDAC6 Mouse Monoclonal Antibody (PCRP-HDAC6-1A4) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
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Additional information

Catalog Number: 10013-MSM1
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human Colon stained with Cadherin 17 / CDH17 Mouse Monoclonal Antibody (CDH17/2617).
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  • IHC
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Additional information

Catalog Number: 1015-MSM7
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human Colon stained with CDH17-Monospecific Mouse Monoclonal Antibody (CDH17/2615).
3
  • IHC
  • WB

Cadherin 17 / LI Cadherin (Gastric Adenocarcinoma Marker) Antibody

Additional information

Catalog Number: 1015-MSM5
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human heart stained with N-Cadherin Recombinant Rabbit Monoclonal Antibody (CDH2/3874R).
3
  • IHC
  • WB

Recombinant N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody

Additional information

Catalog Number: 1000-RBM5
Gene:

Host:

Reactivity:

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Other Information

Functions
  • Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).
Key References
  • Moll, R., et al. 1993. Differentiation 53: 75-93.
  • Wauters, C.C., et al. 1995. Hum. Pathol. 26: 852-855.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
54474
SwissProt
P35900
Chromosome Location
17q21.2
Mol. Weight of Antigen
46kDa
Alternate Names
Keratin, type I cytoskeletal 20, Cytokeratin-20, Keratin-20, Protein IT, CK20; Cytokeratin-20; K20; KA20; Keratin 20; keratin 20, type I; Keratin type I cytoskeletal 20; Keratin-20; KRT20

Summary

This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.

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