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Recombinant Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker) Antibody [KRT20/4879R]

In Stock
Catalog Number Formulation Size Price
54474-RBM9-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
54474-RBM9-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
54474-RBM9-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker) Antibody

IHC analysis of formalin-fixed, paraffin-embedded human brain. Stained with OLIG2/6695R at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary; secondary antibody negative control.

Recombinant Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker) Antibody - Image 2

IHC analysis of formalin-fixed, paraffin-embedded human colon. Stained with KRT20/4879R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker) Antibody - Image 3

SDS-PAGE Analysis of Purified CK20 Recombinant Rabbit Monoclonal Antibody (KRT20/4879R). Confirmation of Purity and Integrity of Antibody.

Cytokeratin 20 Antibody in Western Blot (WB)

Cytokeratin 20 Antibody in Western Blot (WB)

Western Blot Analysis of HT-29 cell lysate using Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody (KRT20/4879R).

Cytokeratin 20 Antibody in Western Blot (WB)

Cytokeratin 20 Antibody in Western Blot (WB)

Western Blot Analysis of Human Intestine and HCT116 lysates using Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody (KRT20/4879R).

Cytokeratin 20 Antibody in Western Blot (WB)

Cytokeratin 20 Antibody in Western Blot (WB)

Western Blot Analysis of Intestine tissue lysates of different species using Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody (KRT20/4879R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Developmental Biology
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm
Gene Name
KRT20
Positive Control
HT-29 cells. Human colon or prostate carcinoma. Human Intestine, HCT116
Immunogen
Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)
Alternate Names
Keratin, type I cytoskeletal 20, Cytokeratin-20, Keratin-20, Protein IT, CK20; Cytokeratin-20; K20; KA20; Keratin 20; keratin 20, type I; Keratin type I cytoskeletal 20; Keratin-20; KRT20

Database Links

Entrez Gene ID
54474
SwissProt
P35900

Additional Information

Clone
KRT20/4879R
Chromosome Location
17q21.2
Mol. Weight of Antigen
46kDa

Related Antibodies

View All KRT20 Antibodies

Functions

  • Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).

Key References

  • Moll, R., et al. 1993. Differentiation 53: 75-93.
  • Wauters, C.C., et al. 1995. Hum. Pathol. 26: 852-855.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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