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Printable Datasheet

Cytokeratin 16 (KRT16) (Suprabasal Keratinocyte Marker) Antibody [SPM264]

In Stock
Catalog Number Formulation Size Price
3868-MSM2X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3868-MSM2X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3868-MSM2X-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Tonsil stained with Cytokeratin 16 Mouse Monoclonal Antibody (SPM264).

Formalin-fixed, paraffin-embedded human Tonsil stained with Cytokeratin 16 Mouse Monoclonal Antibody (SPM264).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Cytokeratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I Cytokeratins (Cytokeratins 9 to 23) and one type II Cytokeratins (keratins 1 to 8). The cytokeratin proteins play a critical role in differentiation, as well as tissue specialization and function, to maintain the overall structural integrity of epithelial cells. Cytokeratins are also useful markers in identifying the origin of metastatic tumors. Cytokeratin 16 is expressed in benign stratified squamous epithelium and squamous cell carcinoma of the head and neck, as well as luminal cells of mammary gland and sweat ducts. It is absent in non-invasive breast carcinomas and normal breast tissue.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Developmental Biology
Isotype / Light Chain
IgG1 / Kappa
Gene Name
KRT16
Positive Control
HeLa cells. Tonsil, Skin. Bladder or Cervix.
Immunogen
Recombinant fragment from the C-terminal of human Cytokeratin 16
Alternate Names
Keratin, type I cytoskeletal 16, Cytokeratin-16, Keratin-16, CK16; Cytokeratin-16; Focal non epidermolytic palmoplantar keratoderma (FNEPPK / NEPPK); K1CP; Keratin Type I Cytoskeletal 16; Keratin-16; KRT16A

Database Links

Entrez Gene ID
3868
SwissProt
P08779

Additional Information

Clone
SPM264
Chromosome Location
17q21.2
Mol. Weight of Antigen
48kDa

Related Antibodies

View All KRT16 Antibodies

Functions

  • Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.

Key References

  • Markey et al. 1992. Br J Dermatol. 126(2):154-60.
  • Wetzels et al. 1991. Am J Pathol. 138(3):751-63.
  • Porter et al. 2000. Lab Invest. 80(11):1701-10.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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