Cytokeratin 16 (KRT16) (Suprabasal Keratinocyte Marker) [KRT16/1714]

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Summary

Cytokeratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I Cytokeratins (Cytokeratins 9 to 23) and one type II Cytokeratins (keratins 1 to 8). The cytokeratin proteins play a critical role in differentiation, as well as tissue specialization and function, to maintain the overall structural integrity of epithelial cells. Cytokeratins are also useful markers in identifying the origin of metastatic tumors. Cytokeratin 16 is expressed in benign stratified squamous epithelium and squamous cell carcinoma of the head and neck, as well as luminal cells of mammary gland and sweat ducts. It is absent in non-invasive breast carcinomas and normal breast tissue.

Product Properties & Targets

Host
Species Reactivity
Research Areas
Isotype
Gene Name
KRT16
Mol. Weight of Antigen
48kDa
Immunogen
Recombinant fragment from the C-terminal of human Cytokeratin 16

Database Links

Entrez Gene ID
Unigene
SwissProt

Additional Information

Clone
KRT16/1714
Human Gene Symbol
KRT16
Chromosome Location
17q21.2

Known Applications & Suggested Dilutions

  • Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
  • Optimal dilution for a specific application should be determined.

Key References

  • Wetzels et al. 1991. Am J Pathol. 138(3):751-63.
  • Markey et al. 1992. Br J Dermatol. 126(2):154-60.
  • Porter et al. 2000. Lab Invest. 80(11):1701-10.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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