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Recombinant CD71 / Transferrin Receptor (TFRC) (Extracellular Domain) Antibody [TFRC/9106R]

In Stock
Catalog Number Formulation Size Price
7037-RBM14-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
7037-RBM14-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
7037-RBM14-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD71 / Transferrin Receptor (TFRC) (Extracellular Domain) Antibody

Formalin-fixed, paraffin-embedded human placenta stained with CD71 Rabbit Recombinant Monoclonal Antibody (TFRC/9106R). Inset: PBS instead of primary antibody; secondary only negative control.

CD71 Antibody in Western Blot (WB)

CD71 Antibody in Western Blot (WB)

Western Blot Analysis of PC-3 cell lysate using CD71 Recombinant Rabbit Monoclonal Antibody (TFRC/9106R).

CD71 Antibody in Western Blot (WB)

CD71 Antibody in Western Blot (WB)

Western blot analyses of (1) human lungs, (2) mouse lungs and (3) rat lungs tissue lysates using CD71 Recombinant Rabbit Monoclonal Antibody (TFRC/9106R).

TFRC Antibody in SDS-PAGE

TFRC Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Transferrin receptor protein 1 | Transferrin receptor protein 1, serum form Recombinant Rabbit Monoclonal Antibody (TFRC/9106R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Signal Transduction
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell surface
Gene Name
TFRC
Positive Control
Human placenta, lungs or bone marrow, PC-3 cell lysate
Immunogen
Recombinant human fragment (around aa50-250) of human TFRC protein (exact sequence is proprietary)
Alternate Names
Transferrin receptor protein 1 | Transferrin receptor protein 1, serum form, T9, p90, Mtvr-1, p90, TFR1, TFRC transferrin receptor (p90 CD71), TRFR

Database Links

Entrez Gene ID
7037
SwissProt
P02786

Additional Information

Clone
TFRC/9106R
Chromosome Location
3q29
Mol. Weight of Antigen
85-95kDa (monomer); 190kDa (dimer)

Related Antibodies

View All TFRC Antibodies

Functions

  • Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes (PubMed:26214738). Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240). Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway (PubMed:26214738). When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion (PubMed:26214738). When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2 (PubMed:26214738).
  • (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.

Key References

  • Oudermans et al. Cancer, 1986; 58:1252.
  • K. Moolenaar et al. Cancer research 50,1102-1106, 1990.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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