Formalin-fixed, paraffin-embedded human tonsil stained with CD2 Recombinant Rabbit Monoclonal Antibody (LFA2/3417R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human tonsil stained with CD2 Recombinant Rabbit Monoclonal Antibody (LFA2/3417R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human lymph node stained with CD2 Recombinant Rabbit Monoclonal Antibody (LFA2/3417R). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using LFA2/3417R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis Purified CD2 Recombinant Rabbit Monoclonal Antibody (LFA2/3417R). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of Jurkat cell lysate using CD2 Recombinant Rabbit Monoclonal Antibody (LFA2/3417R).

Printable Datasheet

CD2 / Lymphocyte Function Antigen 2 (LFA-2) [LFA2/3417R]

Name: CD2 / Lymphocyte Function Antigen 2 (LFA-2)
SKU: 914-RBM7
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

CD2 is mainly present on the surface of mature T cells and NK cell membranes, while B cells are usually not expressed. It can be used to label the diagnosis and study of normal T cells and their associated tumors, such as pre-t cell lymphoma, marginal T cell lymphoma and anaplastic cell lymphoma. CD2 antibody is useful for identification of precursor and mature T-cell lymphomas. Aberrant loss of CD2 in T-cell lymphomas may help to distinguish them from reactive T-cell proliferations.

Product Properties & Targets

Host

Rabbit

Species Reactivity

Human

Isotype

IgG

Cellular Localization

Cell membrane

Gene Name

CD2

Mol. Weight of Antigen

39kDa

Immunogen

Recombinant fragment corresponding to N-terminal of human CD2 protein (exact sequence is proprietary)

Database Links

Entrez Gene ID

914

Unigene

523500

SwissProt

P06729

Additional Information

Clone

LFA2/3417R

Human Gene Symbol

CD2

Chromosome Location

1p13.1

Known Applications & Suggested Dilutions

Western Blot (1-2ug/ml)
,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Companioni N poles O, et al. J Gastroenterol 52:39-49 (2017).
DE Sanctis V, Belgioia L, et al. Anticancer Research 39:1935-1942 (2019).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.