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Recombinant CD2 / Lymphocyte Function Antigen 2 (LFA-2) Antibody [LFA2/3417R]

In Stock
Catalog Number Formulation Size Price
914-RBM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
914-RBM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
914-RBM7-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD2 / Lymphocyte Function Antigen 2 (LFA-2) Antibody

Formalin-fixed, paraffin-embedded human tonsil stained with CD2 Recombinant Rabbit Monoclonal Antibody (LFA2/3417R). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant CD2 / Lymphocyte Function Antigen 2 (LFA-2) Antibody - Image 2

Formalin-fixed, paraffin-embedded human tonsil stained with CD2 Recombinant Rabbit Monoclonal Antibody (LFA2/3417R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Recombinant CD2 / Lymphocyte Function Antigen 2 (LFA-2) Antibody - Image 3

Formalin-fixed, paraffin-embedded human lymph node stained with CD2 Recombinant Rabbit Monoclonal Antibody (LFA2/3417R). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Recombinant CD2 / Lymphocyte Function Antigen 2 (LFA-2) Antibody - Image 4

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using LFA2/3417R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Recombinant CD2 / Lymphocyte Function Antigen 2 (LFA-2) Antibody - Image 5

SDS-PAGE Analysis Purified CD2 Recombinant Rabbit Monoclonal Antibody (LFA2/3417R). Confirmation of Purity and Integrity of Antibody.

Recombinant CD2 / Lymphocyte Function Antigen 2 (LFA-2) Antibody - Image 6

Western blot analysis of Jurkat cell lysate using CD2 Recombinant Rabbit Monoclonal Antibody (LFA2/3417R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

CD2 is mainly present on the surface of mature T cells and NK cell membranes, while B cells are usually not expressed. It can be used to label the diagnosis and study of normal T cells and their associated tumors, such as pre-t cell lymphoma, marginal T cell lymphoma and anaplastic cell lymphoma. CD2 antibody is useful for identification of precursor and mature T-cell lymphomas. Aberrant loss of CD2 in T-cell lymphomas may help to distinguish them from reactive T-cell proliferations.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
AKT Signaling, B Cell Markers, Hematopoietic Stem Cells
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane
Gene Name
CD2
Positive Control
Human tonsil, Spleen or lymph node.
Immunogen
Recombinant fragment corresponding to N-terminal of human CD2 protein (exact sequence is proprietary)
Alternate Names
T-cell surface antigen CD2, Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, T-cell surface antigen T11/Leu-5, Erythrocyte receptor; LFA-2; LFA-3 receptor; Ly-37; Lymphocyte function antigen 2; Rosette receptor; SRBC; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5; T11

Database Links

Entrez Gene ID
914
SwissProt
P06729

Additional Information

Clone
LFA2/3417R
Chromosome Location
1p13.1
Mol. Weight of Antigen
39kDa

Related Antibodies

View All CD2 Antibodies

Functions

  • CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Key References

  • Companioni N poles O, et al. J Gastroenterol 52:39-49 (2017).
  • DE Sanctis V, Belgioia L, et al. Anticancer Research 39:1935-1942 (2019).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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