Formalin-fixed, paraffin-embedded human colon carcinoma stained with CEA Mouse Recombinant Monoclonal Antibody (rC66/1009).

SDS-PAGE Analysis Purified CEA Mouse Recombinant Monoclonal Antibody (rC66/1009). Confirmation of Purity and Integrity of Antibody.

Printable Datasheet

Recombinant Carcinoembryonic Antigen (CEA) / CD66 [rC66/1009]

Name: Carcinoembryonic Antigen (CEA) / CD66
SKU: 1048-MSM14
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies Recombinant Mouse Monoclonals Recombinants

Summary

This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.

Product Properties & Targets

Host

Mouse

Species Reactivity

Human

Isotype

IgG1

Cellular Localization

Apical cell membrane, Cell membrane, Cell surface

Light Chain

kappa

Gene Name

CEACAM5

Positive Control

MCF7 or 293T cells. Human colon carcinoma.

Immunogen

Recombinant full-length human CEA protein

Alternate Names

Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen, Meconium antigen 100, Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66, Biliary Glycoprotein (BGP-1)

Database Links

Entrez Gene ID

1048

Unigene

709196

SwissProt

P06731

Additional Information

Clone

rC66/1009

Human Gene Symbol

CEACAM5

Chromosome Location

19q13.2

Mol. Weight of Antigen

80-200kDa

Functions

Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed:2803308, PubMed:10910050, PubMed:10864933). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:2803308). Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells (PubMed:10910050).
(Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.

Known Applications & Suggested Dilutions

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Muraro R, et. al. Cancer Research, 1985, 45:5769-80.
Siler K, et. al. Biotechnology Therapeutics, 1993, 4(3-4):163-81.
Robbins PF, et. al. International Journal of Cancer, 1993, 53(6):892-7.
Shi ZR, et. al. Journal of Histochemistry and Cytochemistry, 1994, 42(9):1215-9.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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