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Recombinant Carcinoembryonic Antigen (CEA) / CD66e Antibody [C66/3707R]

Additional information

Catalog Number: 1048-RBM17
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
1048-RBM17-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
1048-RBM17-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1048-RBM17-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Carcinoembryonic Antigen (CEA) / CD66e Antibody

Formalin-fixed, paraffin-embedded human colon stained with CEA Recombinant Rabbit Monoclonal Antibody (C66/3707R).

Recombinant Carcinoembryonic Antigen (CEA) / CD66e Antibody - Image 2

Formalin-fixed, paraffin-embedded human colon stained with CEA Recombinant Rabbit Monoclonal Antibody (C66/3707R).

CEA Antibody in Western Blot (WB)

CEA Antibody in Western Blot (WB)

Western Blot Analysis of Human Intestine tissue lysate using CEA Recombinant Rabbit Monoclonal Antibody (C66/3707R).

CEA Antibody in Western Blot (WB)

CEA Antibody in Western Blot (WB)

Western Blot Analysis of Intestine tissue lysates of different species using CEA Recombinant Rabbit Monoclonal Antibody (C66/3707R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
C66/3707R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Apical cell membrane, Cell membrane, Cell surface
Gene Name
CEACAM5
Positive Control
MCF7 or 293T cells. Colon carcinoma. Human Intestine.
Immunogen
Recombinant full-length human CEA protein
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed:2803308, PubMed:10910050, PubMed:10864933). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:2803308). Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells (PubMed:10910050).
  • (Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.
Key References
  • Boogerd, L., et al. 2017. Correlation between preoperative serum carcinoembryonic antigen levels and expression on pancreatic and rectal cancer tissue. Biomark. Cancer 9: 1179299X17710016.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
1048
SwissProt
P06731
Chromosome Location
19q13.2
Mol. Weight of Antigen
180-200kDa
Alternate Names
Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen, Meconium antigen 100, Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66, Biliary Glycoprotein (BGP-1)

Summary

Carcinoembryonic antigen (CEA) is a glycoprotein produced in colorectal cancer tissues. In normal adult tissue, CEA is expressed in the apical border and, to a lesser extent in the cytoplasm, of the columnar cells of colon, small intestine, stomach (surface epithelium, mucous neck cells and weakly in pyloric mucous cells), pancreatic ducts, secretory epithelia of sweat glands, squamous epithelial cells of the tongue, esophagus,uterine cervix, and urothelium. The CEA antibody is a useful aid for classification of adenocarcinomas, notably in the gastrointestinal tract, including colonic and pancreatic carcinomas. IHC results also aid in the classification of secretory meningiomas and medullary carcinoma of the thyroid.

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