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BARX1 (Prognostic Biomarker in Hepatocellular Carcinoma) Antibody [BARX1/2759]

In Stock
Catalog Number Formulation Size Price
56033-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
56033-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
56033-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Pancreas stained with BARX1 Mouse Monoclonal Antibody (BARX1/2759).

Formalin-fixed, paraffin-embedded human Pancreas stained with BARX1 Mouse Monoclonal Antibody (BARX1/2759).

Formalin-fixed, paraffin-embedded human Pancreas stained with BARX1 Mouse Monoclonal Antibody (BARX1/2759).

Formalin-fixed, paraffin-embedded human Pancreas stained with BARX1 Mouse Monoclonal Antibody (BARX1/2759).

SDS-PAGE Analysis of Purified BARX1 Mouse Monoclonal Antibody (BARX1/2759). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified BARX1 Mouse Monoclonal Antibody (BARX1/2759). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

BarX1 is a member of the Bar subclass of homeobox transcription factors. Studies of the Mouse and chick homolog suggest the encoded protein may play a role in developing teeth and craniofacial mesenchyme of neural crest origin. The protein may also be associated with differentiation of stomach epithelia. Down-regulation of Barx1 promotes HCC migration, invasion and metastasis, whereas up-regulation of Barx1 inhibits HCC migration, invasion and metastasis. The loss of Barx1 expression represents a prognostic biomarker in human HCC.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Nuclear Marker
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane, Secreted
Gene Name
MUC4
Positive Control
HepG2 cell lysate. Pancreas.
Immunogen
Recombinant fragment of human BARX1 protein (around aa 155-254) (exact sequence is proprietary)
Alternate Names
Mucin-4, Ascites sialoglycoprotein, Pancreatic adenocarcinoma mucin, Testis mucin, Tracheobronchial mucin, BarH like homeobox 1; BarX 1; BARX homeobox 1; Homeobox protein BarH like 1

Database Links

Entrez Gene ID
56033
SwissProt
Q9HU1

Additional Information

Clone
BARX1/2759
Chromosome Location
9q22.32
Mol. Weight of Antigen
24kDa

Related Antibodies

View All MUC4 Antibodies

Functions

  • Membrane-bound mucin, a family of highly glycosylated proteins that constitute the major component of the mucus, the slimy and viscous secretion covering epithelial surfaces (PubMed:10880978). These glycoproteins play important roles in the protection of the epithelium and are implicated in epithelial renewal and differentiation (PubMed:10880978). Regulates cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and its ability to act as an intramembrane ligand for ERBB2. Plays an important role in proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. In polarized epithelial cells, segregates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. Its ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation.

Key References

  • Wang, G., et al. Oncotarget. 2017 Sep 22; 8(42): 71867-71880.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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