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Aldo-keto Reductase Family 1 Member C1 / DD1 Antibody [AKR1C1/9070]

In Stock
Catalog Number Formulation Size Price
1645-MSM10-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1645-MSM10-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1645-MSM10-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

DDH / AKR1C1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cytoplasm.
Gene Name
Positive Control
Human liver or stomach tissue.
Immunogen
Recombinant human full-length AKR1C1 protein
Alternate Names
Aldo-keto reductase family 1 member C1, 20-alpha-hydroxysteroid dehydrogenase, Chlordecone reductase homolog HAKRC, Dihydrodiol dehydrogenase 1, High-affinity hepatic bile acid-binding protein, 2-dihydrobenzene-1; 2-diol dehydrogenase; 20 alpha (3 alpha) hydroxysteroid dehydrogenase; 20ALPHAHSD; 2ALPHAHSD; AK1C1; C9; Chlordecone reductase homolog HAKRC; DD1/DD2; DDH1; Dihydrodiol dehydrogenase 1/2; H37; HBAB; Hepatic dihydrodiol dehydrogenase; High affinity hepatic bile acid-binding protein; Indanol dehydrogenase; MBAB; Trans-1,2 dihydrobenzene 1,2 diol dehydrogenase; Type II 3 alpha hydroxysteroid dehydrogenase

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
AKR1C1/9070
Chromosome Location
10p15.1
Mol. Weight of Antigen
37kDa

Functions

  • Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids (PubMed:19218247). Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH (PubMed:14672942). Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens (PubMed:10998348). May also reduce conjugated steroids such as 5alpha-dihydrotestosterone sulfate (PubMed:19218247). Displays affinity for bile acids (PubMed:8486699).

Key References

  • Binstock, J.M., et al. 1992. Human hepatic 3α-hydroxysteroid dehydrogenase: possible identity with human hepatic chlordecone reductase. Biochem. Biophys. Res. Commun. 187: 760-766.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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