|Catalog No||Formulation||Size||Price (USD)|
|7124-MSM12-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||199.00|
|7124-MSM12-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||429.00|
|7124-MSM12-P1BX||Purified Ab WITHOUT BSA at 1.0mg/ml||100 ug||429.00|
|Human Entrez Gene ID||7124|
|Human Gene Symbol||TNF|
|Human Chromosome Location||6p21.33|
|Synonyms||APC1, Cachectin, Differentiation inducing factor (DIF), Macrophage cytotoxic factor (MCF), Necrosin, TNF alpha, TNF Macrophage Derived, TNF Monocyte Derived, TNF Superfamily Member 2, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor Precursor|
|Immunogen||Recombinant full-length human TNF-alpha protein|
|Host / Ig Isotype||Mouse / IgM, kappa|
|Mol. Weight of Antigen||17kDa|
|Cellular Localization||Cytoplasmic and extracellular (secreted)|
|Species Reactivity||Human. Rat. Others-not known.|
This MAb recognizes human 17-26kDa protein, which is identified as cytokine TNF-alpha (Tumor Necrosis Factor-alpha). TNF-alpha can be expressed as a 17kDa free molecule, or as a 26kDa membrane protein. TNF-alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunohistology (Formalin-paraffin) (2-4ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
HeLa, HL-60, or A431 cells. Macrophages in lymph node or tonsil or histiocytoma tissue.
200ug/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.