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Home >> Antibodies >> TNF-alpha (Tumor Necrosis Factor alpha)

TNF-alpha (Tumor Necrosis Factor alpha)

Mouse Monoclonal Antibody [Clone TNFA/1172]

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Catalog No Formulation Size Price (USD)  
7124-MSM12-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
7124-MSM12-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 429.00
7124-MSM12-P1BX Purified Ab WITHOUT BSA at 1.0mg/ml 100 ug 429.00
Human Entrez Gene ID 7124
Human SwissProt P01375
Human Unigene 241570
Human Gene Symbol TNF
Human Chromosome Location 6p21.33
Synonyms APC1, Cachectin, Differentiation inducing factor (DIF), Macrophage cytotoxic factor (MCF), Necrosin, TNF alpha, TNF Macrophage Derived, TNF Monocyte Derived, TNF Superfamily Member 2, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor Precursor
Immunogen Recombinant full-length human TNF-alpha protein
Host / Ig Isotype Mouse / IgM, kappa
Mol. Weight of Antigen 17kDa
Cellular Localization Cytoplasmic and extracellular (secreted)
Species Reactivity Human. Rat. Others-not known.

Specificity & Comments

This MAb recognizes human 17-26kDa protein, which is identified as cytokine TNF-alpha (Tumor Necrosis Factor-alpha). TNF-alpha can be expressed as a 17kDa free molecule, or as a 26kDa membrane protein. TNF-alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-paraffin) (2-4ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

HeLa, HL-60, or A431 cells. Macrophages in lymph node or tonsil or histiocytoma tissue.

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.


This antibody is available for research use only and is not approved for use in diagnosis.


There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Bebok Z; Markus B; Nemeth P. Prognostic relevance of transforming growth factor alpha (TGF-alpha) and tumor necrosis factor alpha (TNF-alpha) detected in breast cancer tissues by immunohistochemistry. Breast Cancer Research and Treatment, 1994, 29(3):229-35.