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Home >> Antibodies >> TNF-alpha (Tumor Necrosis Factor alpha)

TNF-alpha (Tumor Necrosis Factor alpha)

Mouse Monoclonal Antibody [Clone 4C6-H8]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Histiocytoma stained with TNF alpha Mouse Monoclonal Antibody (4C6-H8).

img

Immunofluorescence staining of paraformaldehyde-fixed HePG2 cells with TNF alpha Mouse Monoclonal Antibody (4C6-H8) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Reddot (Red).

Product Details

Synonyms

APC1, Cachectin, Differentiation inducing factor (DIF), Macrophage cytotoxic factor (MCF), Necrosin, TNF alpha, TNF Macrophage Derived, TNF Monocyte Derived, TNF Superfamily Member 2, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor Precursor

Positive Control

HeLa, HL-60 or A431 cells. Macrophages in lymph node or Tonsil.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

A hexadecapeptide corresponding to aa115-130 (NGVELRDNQLVVPSEG) of human TNF- , conjugated to thyroglobulin

Cellular Localization

Cytoplasmic and extracellular (secreted)

Species Reactivity

Human. Mouse. Rat. Rabbit. Cat. Dog. Zebrafish.

Host / Ig Isotype

Mouse / IgM, kappa

Mol. Weight of Antigen

17kDa

Specificity & Comments

Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

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200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Bebok Z, et al. Orvosi Hetilap, 1993 Jun 13, 134(24):1303-7. Language: Hungarian.

Bioinformatics

  •  Entrez Gene ID: 7124
  •  Gene Symbol: TNF
  •  SwissProt: P01375
  •  Chromosome Location: 6p21.33
  •  Unigene: 241570
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