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Home >> Antibodies >> SOX2 (Embryonic Stem Cell Marker)

SOX2 (Embryonic Stem Cell Marker)

Mouse Monoclonal Antibody [Clone SOX2/1791]

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Catalog No Formulation Size Price (USD)  
6657-MSM1-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
6657-MSM1-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 429.00
6657-MSM1-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 429.00
Human Entrez Gene ID 6657
Human SwissProt P48431
Human Unigene 518438
Human Gene Symbol SOX2
Human Chromosome Location 3q26.33
Synonyms ANOP3; Delta EF2a; MCOPS3 (Microphthalmia Syndromic type 3); SOX-2; SRY (sex determining region Y) box 2; SRY related HMG box 2; Transcription factor SOX-2; ysb
Immunogen Recombinant fragment (within aa176-305) of human SOX2 protein (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG2b, kappa
Mol. Weight of Antigen 34kDa
Cellular Localization Nuclear
Species Reactivity Human and Mouse. Others not known.

Specificity & Comments

SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. Reportedly, SOX2 is associated with aggressive phenotypes of breast, head and neck, gastric, colorectal, bladder, and small cell lung cancers. However, SOX2 is expressed in a high percentage of lung squamous cell carcinomas and has been shown to be an independent favorable prognostic marker.

Known Applications & Suggested Dilutions

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Western Blotting (1-2ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

MCF-7 cells. Lung or Cervical carcinoma

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. ,Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Laudet, V., Stehelin, D. and Clevers, H. 1993. Nucleic Acids Res. 21: 2493-2501
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