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Home >> Antibodies >> p27Kip1 (Mitotic Inhibitor/Suppressor Protein)

p27Kip1 (Mitotic Inhibitor/Suppressor Protein)

Mouse Monoclonal Antibody [Clone DCS-72.F6]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Cervical Cancer stained with p27 Monoclonal Antibody (DCS-72.F6)

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Formalin-fixed, paraffin-embedded human Colon Cancer stained with p27 Monoclonal Antibody (DCS-72.F6)

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Flow Cytometry of human p27 on HeLa cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled p27 Monoclonal Antibody (DCS-72.F6).

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SDS-PAGE Analysis Purified p27 MAb (DCS-72.F6). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, Cyclin-dependent kinase inhibitor p27 Kip1, KIP1, MEN1B, MEN4

Positive Control

ZR75, T47D, SK-BR-3, MDA-MB-231, HeLa or MCF7 cells. Tonsil, Breast, Cervical or Colon Carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length Mouse p27 protein

Cellular Localization

Nuclear

Species Reactivity

Human. Monkey. Mouse. Rat.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

25-26kDa

Specificity & Comments

Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Fredersdorf S et. al. Proc Natl Acad Sci 1997;94:6380-5.

Bioinformatics

  •  Entrez Gene ID: 1027
  •  Gene Symbol: CDKN1B
  •  SwissProt: P46527
  •  Chromosome Location: 12p13.1
  •  Unigene: 238990
NBT00771