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Home >> Antibodies >> MLH1 (MutL Homolog 1) / HNPCC

MLH1 (MutL Homolog 1) / HNPCC

Recombinant Mouse Monoclonal Antibody [Clone MLH1/6467]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

IHC analysis of FFPE Lynch Syndrome / Hereditary Non-Polyposis Colorectal Cancer (HNPCC). MLH1/6467at 2ug/ml in PBS, 30 min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.Inset: PBS instead of primary, secondary control.

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Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with MLH1 / MutL Homolog 1 Mouse Monoclonal Antibody (MLH1/6467).

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SDS-PAGE Analysis Purified MLH1 Mouse Monoclonal Antibody (MLH1/6467). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

COCA2; DNA mismatch repair protein; FCC2; hMLH1; MutL homolog 1 colon cancer nonpolyposis type 2 (HNPCC2)

Positive Control

Human tonsil or colon carcinoma.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human MLH1 protein

Cellular Localization

Nuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

85kDa

Specificity & Comments

This MAb recognizes a protein of 83kDa, identified as MLH1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2).Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process, which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma, which plays a role in meiosis.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. R schle M, et al. J Biol Chem.1999; 274:32368-75.
  2. Garg K, et al. Am J Surg Pathol. 2009; 33(6):925-33.

Bioinformatics

  •  Entrez Gene ID: 4292
  •  Gene Symbol: MLH1
  •  SwissProt: P40692
  •  Chromosome Location: 3p21.3
  •  Unigene: 195364