Home >> Antibodies >> HSP60 (Heat Shock Protein 60) (Mitochondrial Marker)

HSP60 (Heat Shock Protein 60) (Mitochondrial Marker)

Mouse Monoclonal Antibody [Clone AE-1]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with HSP60 Mouse Monoclonal Antibody (AE-1).

Immunofluorescent staining of PFA-fixed HeLa cells. HSP60 Monoclonal Antibody (AE-1) (CF488). Nuclei stained with RedDot (red).

Western blot analysis of Hep-2 and Hela cell lysate using HSP60 MAb (LK1)

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with HSP60 Mouse Monoclonal Antibody (AE-1).

Immunofluorescent staining of PFA-fixed HeLa cells. HSP60 Monoclonal Antibody (AE-1) (CF488). Nuclei stained with RedDot (red).

Western blot analysis of Hep-2 and Hela cell lysate using HSP60 MAb (LK1)

Product Details

Synonyms

60kDa chaperonin, 60kDa heat shock protein mitochondrial, Chaperonin, 60-KD (CPN60), GROEL, HLD4, HSP65, HSPD1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, Short heat shock protein 60 Hsp60s1, Spastic paraplegia 13 (SPG13)

Positive Control

HeLa or HepG2 cells. Breast carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human HSPD1 protein

Cellular Localization

Mitochondrial

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

60kDa

Specificity & Comments

Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). Hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.